| Full text | |
| Author(s): |
de Jesus, Adriana Almeida
[1]
;
Arruda Campos, Lucia Maria
[1]
;
Liphaus, Bernadete Lourdes
[2]
;
Carneiro-Sampaio, Magda
[3]
;
Pitangueira Mangueira, Cristovao Luis
[4, 5]
;
Rosseto, Eliane Aparecida
[4, 5]
;
Almeida da Silva, Clovis Artur
[6, 7]
;
Scheinberg, Morton
Total Authors: 8
|
| Affiliation: | [1] Univ Sao Paulo ICr HC FMUSP, Fac Med, Hosp Clin, Pediat Rheumatol Unit, Inst Crianca, Sao Paulo - Brazil
[2] HC FMUSP, Med Invest Lab MIL 36, Sao Paulo - Brazil
[3] HC FMUSP, Dept Rheumatol, Sao Paulo - Brazil
[4] HC FMUSP, Dept Pathol, Hosp Israelita Albert Einstein, Sao Paulo - Brazil
[5] HC FMUSP, Cent Lab, Autoantibodies Sect, Sao Paulo - Brazil
[6] HC FMUSP, Dept Pediat, Sao Paulo - Brazil
[7] HC FMUSP, Rheumatol Unit, Sao Paulo - Brazil
Total Affiliations: 7
|
| Document type: | Journal article |
| Source: | REVISTA BRASILEIRA DE REUMATOLOGIA; v. 52, n. 6, p. 971-981, NOV-DEC 2012. |
| Web of Science Citations: | 1 |
| Abstract | |
OBJECTIVES: To evaluate the presence of anti-C1q, anti-chromatin/nucleosome and anti-double stranded DNA (dsDNA) antibodies in juvenile systemic lupus erythematosus (JSLE) and controls. METHODS: Sixty-seven JSLE and 34 healthy controls were analyzed for the presence of anti-C1q, anti-chromatin/nucleosome, and anti-dsDNA antibodies by ELISA. C1q levels were evaluated by radial immunodiffusion. RESULTS: The mean current age was similar in JSLE patients and controls (14.6 ± 3.86 vs. 13.6 ± 2.93 years, P = 0.14). Higher frequencies of anti-C1q, anti-chromatin/nucleossome, and anti-dsDNA antibodies were observed in JSLE compared to controls (20% vs. 0%, P = 0.0037; 48% vs. 0%, P < 0.0001 and 69% vs. 3%, P < 0.0001, respectively). The median of anti-C1q, anti-chromatin/nucleossome, and antidsDNA antibodies were also significantly higher in JSLE patients than in controls [9.6 (5.5-127) vs. 7.5 (5-20) units, P = 0.0006; 18 (1.9-212) vs. 3.2 (1.7-17) units, P < 0.0001; and 111 IU/mL (6-741) vs. 14 (6-33) IU/mL; P < 0.0001, respectively]. The sensitivity for anti-C1q, anti-chromatin/nucleosome, and anti-dsDNA antibodies was 21% (CI: 11-33), 49% (CI: 36-62), and 70% (CI: 57-81). The specificity was 100% (CI: 88-100), 100% (88-100), and 97% (CI: 83-99), respectively. A positive correlation was found between anti-dsDNA levels and both anti-C1q (r = 0.51; CI: 0.29-0.68; P < 0.0001) and anti-chromatin/nucleosome antibodies (r = 0.87; CI: 0.79-0.92; P < 0.0001) levels. A negative correlation was observed between anti-C1q and C1q levels (r = -0.33; CI: -0.56-0.05; P = 0.018). The frequency of anti-dsDNA was higher in patients with SLEDAI-2K >1 (P = 0.0047) and no differences were observed in the frequencies of these three autoantibodies and nephritis (P > 0.05). CONCLUSION: Our study demonstrated an elevated specificity for lupus diagnosis involving the three autoantibodies, especially anti-C1q and anti-chromatin/nucleosome. (AU) | |
| FAPESP's process: | 08/58238-4 - Autoimmunity in children: investigation of the molecular and cellular bases of early onset of autoimmunity |
| Grantee: | Magda Maria Sales Carneiro-Sampaio |
| Support Opportunities: | Research Projects - Thematic Grants |