Glycolaldehyde impairs neutrophil biochemical parameters by an oxidative and calcium-dependent mechanism-Protective role of antioxidants astaxanthin and vitamin C

Author(s)

Guerra, Beatriz Alves ^[1] ; Bolin, Anaysa Paola ^[1] ; Morandi, Ana Carolina ^[1] ; Otton, Rosemari ^[1]

Affiliation

^[1] Univ Cruzeiro Sul, CBS, Postgrad Program, BR-03342000 Sao Paulo - Brazil

• Document type: Journal article
• Source: Diabetes Research and Clinical Practice; v. 98, n. 1, p. 108-118, OCT 2012.
• Web of Science Citations: 9

Abstract

Aim: The present study examined the effects of glycolaldehyde (GC) on biochemical parameters of human neutrophils and whether the antioxidant astaxanthin associated with vitamin C can modulate these parameters. Methods: Neutrophils from healthy subjects were treated with GC (1 mM) followed or not by the antioxidants astaxanthin (2 mu M) and vitamin C (100 mu M). We examined the phagocytic capacity, hypochlorous acid, myeloperoxidase (MPO) and glucose-6-phosphate dehydrogenase (G6PDH) activities, cytokines and {[}Ca2+](i). Also, superoxide anion, hydrogen peroxide, nitric oxide production, antioxidant enzyme activities and glutathione-recycling system were evaluated. Results: GC promoted a marked reduction on the phagocytic capacity, maximal G6PDH and MPO activities, hypochlorous acid production and release of IL-1 beta, IL-6 and TNF-alpha cytokines. Some impairment in the neutrophils biochemical parameters appears to be mediated by oxidative stress through ROS/RNS production and calcium reduction. Oxidative stress was evidenced by reduction in the activities of the main antioxidant enzymes, GSH/GSSG ratio and in the increment of O-2(center dot) and H2O2 and N-O(center dot). Conclusions: Treatment of cells with the combination of the antioxidants astaxanthin and vitamin C was able to restore some neutrophils function mainly by decreasing ROS/RNS production and improving the redox state. Overall, our findings demonstrate that GC modulates several neutrophils biochemical parameters in vitro. (C) 2012 Elsevier Ireland Ltd. All rights reserved. (AU)

• FAPESP's process: 09/14382-7 - Effect of methylglyoxal and glucose on the function of human leukocytes: possible protective role of astaxantin and vitamin C
• Grantee: Rosemari Otton
• Support Opportunities: Regular Research Grants
• FAPESP's process: 09/17381-1 - Addition of glucose and methylglyoxal on neutrophil function: role of astaxanthin and vitamin C
• Grantee: Beatriz Alves Guerra
• Support Opportunities: Scholarships in Brazil - Scientific Initiation