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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Mast Cells Act as Phagocytes Against the Periodontopathogen Aggregatibacter Actinomycetemcomitans

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Author(s):
Lima, Heliton G. [1] ; Pinke, Karen H. [1] ; Gardizani, Taiane P. [1] ; Souza-Junior, Devandir A. [2] ; Carlos, Daniela [3] ; Avila-Campos, Mario J. [4] ; Lara, Vanessa S. [1]
Total Authors: 7
Affiliation:
[1] Univ Sao Paulo, Bauru Sch Dent, Dept Stomatol, BR-17012901 Bauru, SP - Brazil
[2] Univ Sao Paulo, Dept Cell & Mol Biol & Pathogen Bioagents, Sch Med Ribeirao Preto, BR-14049 Ribeirao Preto, SP - Brazil
[3] Univ Sao Paulo, Dept Biochem & Immunol, Sch Med Ribeirao Preto, BR-05508 Sao Paulo - Brazil
[4] Univ Sao Paulo, Dept Microbiol, Inst Biomed Sci, Sao Paulo - Brazil
Total Affiliations: 4
Document type: Journal article
Source: Journal of Periodontology; v. 84, n. 2, p. 265-272, FEB 2013.
Web of Science Citations: 8
Abstract

Background: Evidence to date shows that mast cells play a critical role in immune defenses against infectious agents, but there have been no reports about involvement of these cells in eliminating periodontopathogens. In this study, the phagocytic ability of mast cells against Aggregatibacter actinomycetemcomitans compared with macrophages is evaluated. Methods: In vitro phagocytic assays were conducted using murine mast cells and macrophages, incubated with A. actinomycetemcomitans, either opsonized or not, with different bacterial load ratios. After 1 hour, cells were stained with acridine orange and assessed by confocal laser-scanning electron microscopy. Results: Phagocytic ability of murine mast cells against A. actinomycetemcomitans was confirmed. In addition, the percentage of mast cells with internalized bacteria was higher in the absence of opsonization than in the presence of opsonization. Both cell types showed significant phagocytic activity against A. actinomycetemcomitans. However, the percentage of mast cells with non-opsonized bacteria was higher than that of macrophages with opsonized bacteria in one of the ratios (1:10). Conclusions: This is the first report about the participation of murine mast cells as phagocytes against A. actinomycetemcomitans, mainly in the absence of opsonization with human serum. Our results may indicate that mast cells act as professional phagocytes in the pathogenesis of biofilmassociated periodontal disease. J Periodontol 2013;84:265-272. (AU)

FAPESP's process: 09/14152-1 - Defense mechanisms of mast cells against Candida albicans: involvement of Toll-like 2 receptor in the production of cytokines TNF-alpha and IL-10, and the phagocytic and antimicrobial activities
Grantee:Vanessa Soares Lara
Support Opportunities: Regular Research Grants