Filamentous fungi from the Atlantic marine sponge Dragmacidon reticulatum

Author(s)

Passarini, Michel R. Z. ^{[1, 2]} ; Santos, Cledir ^[2] ; Lima, Nelson ^[2] ; Berlinck, Roberto G. S. ^[3] ; Sette, Lara D. ^{[1, 4]}

Affiliation

^[1] Univ Estadual Campinas, UNICAMP, CPQBA, DRM, Campinas, SP - Brazil

^[2] Univ Minho, Ctr Engn Biol, IBB, Braga - Portugal

^[3] Univ Sao Paulo, Inst Quim Sao Carlos, Sao Carlos, SP - Brazil

^[4] Univ Estadual Sao Paulo, UNESP, Inst Biociencias, Dept Bioquim & Microbiol, BR-13506900 Rio Claro, SP - Brazil

• Document type: Journal article
• Source: Archives of Microbiology; v. 195, n. 2, p. 99-111, FEB 2013.
• Web of Science Citations: 41

Abstract

Dragmacidon reticulatum is a marine sponge of wide occurrence in the Eastern and Western Atlantic. Little is known about D. reticulatum fungal diversity. Filamentous fungi recovered from D. reticulatum were assessed in the present study using a polyphasic taxonomic approach, including classical morphology, molecular biology and MALDI-TOF ICMS. Ninety-eight fungal strains were isolated from two D. reticulatum samples by using six different culture media, which were identified up to the genus level. Sixty-four distinct fungal ribotypes were obtained, distributed among twenty-four different genera belonging to the Ascomycota and Zygomycota. Representatives of Penicillium and Trichoderma were the most diverse and abundant fungi isolated. Amongst Penicillium spp. three isolates belonged to the same ribotype can be considered as a putative new species. Data derived from the present study highlight the importance of using a polyphasic approach to get an accurate identification in order to structure a reliable culture collection. (AU)

• FAPESP's process: 08/06720-7 - Characterization of filamentous fungi diversity from marine sponges and evaluation of laccase production
• Grantee: Michel Rodrigo Zambrano Passarini
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 10/50190-2 - Investigation of metabolic and biotechnological potential of marine organisms in bioremediation processes and for the production of substances with anti-viral, anti-inflammatory and anti-Leishmania activities (Marine Biodiversity)
• Grantee: Roberto Gomes de Souza Berlinck
• Support Opportunities: BIOTA-FAPESP Program - Thematic Grants