| Full text | |
| Author(s): |
Kwiatkoski, Marcelo
[1]
;
Soriano, Renato N.
[2]
;
Araujo, Rebeca M.
[3]
;
Azevedo, Leopoldo U.
[3]
;
Batalhao, Marcelo E.
[3]
;
Francescato, Heloisa D. C.
[1]
;
Coimbra, Terezila M.
[1]
;
Carnio, Evelin C.
[2]
;
Branco, Luiz G. S.
[3]
Total Authors: 9
|
| Affiliation: | [1] Med Sch Ribeirao Preto, BR-14049900 Ribeirao Preto - Brazil
[2] Nursing Sch Ribeirao Preto, BR-14040902 Ribeirao Preto - Brazil
[3] Univ Sao Paulo, Dent Sch Ribeirao Preto, BR-14040904 Ribeirao Preto - Brazil
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | Experimental Neurology; v. 240, p. 88-95, FEB 2013. |
| Web of Science Citations: | 19 |
| Abstract | |
Hydrogen sulfide (H2S) is a gaseous neuromodulator endogenously produced in the brain by the enzyme cystathionine beta-synthase (CBS). We tested the hypothesis that H2S acts within the anteroventral preoptic region of the hypothalamus (AVPO) modulating the production of prostaglandin (PG) E-2 (the proximal mediator of fever) and cyclic AMP (cAMP). To this end, we recorded deep body temperature (Tb) of rats before and after pharmacological modulation of the CBS-H2S system combined or not with lipopolysaccharide (LPS) exposure, and measured the levels of H2S, cAMP, and PGE(2) in the AVPO during systemic inflammation. Intra-cerebroventricular (icy) microinjection of aminooxyacetate (AOA, a CBS inhibitor: 100 pmol) did not affect basal PGE(2) production and Tb, but enhanced LPS-induced PGE(2) production and fever, indicating that endogenous 1125 plays an antipyretic role. In agreement, icy microinjection of a H2S donor (Na2S; 260 nmol) reduced the LPS-induced PGE(2) production and fever. Interestingly, we observed that the AVPO levels of H2S were decreased following the immunoinflammatory challenge. Furthermore, fever was associated with decreased levels of AVPO cAMP and increased levels of AVPO PGE(2). The LPS-induced decreased levels of cAMP were reduced to a lesser extent by the H2S donor. The LPS-induced PGE(2) production was potentiated by AOA (the CBS inhibitor) and inhibited by the H2S donor. Our data are consistent with the notion that the gaseous messenger H2S synthesis is downregulated during endotoxemia favoring PGE(2) synthesis and lowering cAMP levels in the preoptic hypothalamus. (C) 2012 Elsevier Inc. All rights reserved. (AU) | |
| FAPESP's process: | 10/17071-0 - Hydrogen sulfide (H2S) in the thermoregulation and fever |
| Grantee: | Luiz Guilherme de Siqueira Branco |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 11/07043-1 - Role of Hydrogen Sulfide (H2S) in thermoregulation and fever |
| Grantee: | Leopoldo Ulian Azevedo |
| Support Opportunities: | Scholarships in Brazil - Scientific Initiation |
| FAPESP's process: | 11/07257-1 - ROLE OF HYDROGEN SULFIDE (H2S) IN THE NEUROCHEMISTRY OF THERMOREGULATION |
| Grantee: | Rebeca Miranda de Araújo |
| Support Opportunities: | Scholarships in Brazil - Scientific Initiation |