Evaluation of different irrigation protocols concerning the formation of chemical smear layer

Author(s)

Do Prado, Maira ; Simao, Renata A. ^[1] ; Gomes, Brenda P. F. A. ^[2]

Affiliation

^[1] Univ Fed Rio de Janeiro, Dept Met & Mat Engn, Rio De Janeiro, RJ - Brazil

^[2] Univ Estadual Campinas, Piracicaba Dent Sch, Dept Restorat Dent, Endodont Div, UNICAMP, BR-13414018 Piracicaba, SP - Brazil

• Document type: Journal article
• Source: MICROSCOPY RESEARCH AND TECHNIQUE; v. 76, n. 2, p. 196-200, FEB 2013.
• Web of Science Citations: 6

Abstract

The aim of this work was to evaluate, by means of scanning electron microscopy (SEM) and electron dispersive spectroscopy (EDS), the different irrigation protocols concerning the formation of chemical smear layer (CSL). Fifty-five teeth were instrumented by using distilled water (DW) as irrigant. Next, the teeth were submitted to a protocol for removal of the mechanical smear layer produced. The teeth were divided into 11 groups, according to irrigation regimen. Then, the teeth were prepared and analyzed with SEM and EDS. Only in Group 1 {[}1 mL sodium hypochlorite (NaOCl) + 10 mL DW + 1 mL chlorhexidine (CHX) solution], Group 2 {[}1 mL NaOCl + 10 mL DW + 1 mL ethylenediaminetetraacetic acid (EDTA) + 10 mL DW + 1 mL CHX solution], and Group 3 {[}1 mL NaOCl + 10 mL DW + 1 mL citric acid (CA) + 10 mL DW + 1 mL CHX solution], the formation of CSL was observed. In the groups where CHX gel was used alone or in association with EDTA, CA, phosphoric acid (PA), and CHX solution, no formation of CSL was observed. According to the results found in the cases in which one wants to associate NaOCl with CHX, the protocol using PA (1 mL NaOCl + 10 mL DW + 1 mL PA + 10 mL DW + 1 mL CHX solution) did not induce formation of CSL. Microsc. Res. Tech., 2013. (C) 2012 Wiley Periodicals, Inc. (AU)