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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Effect of topical chamomile on immunohistochemical levels of IL-1 beta and TNF-alpha in 5-fluorouracil-induced oral mucositis in hamsters

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Author(s):
Curra, Marina [1] ; Martins, Marco Antonio T. [1] ; Lauxen, Isabel S. [1] ; Pellicioli, Ana Carolina A. [1] ; Sant'Ana Filho, Manoel [1] ; Pavesi, Vanessa Christina S. [2] ; Carrard, Vinicius C. [1] ; Martins, Manoela D. [1]
Total Authors: 8
Affiliation:
[1] Univ Fed Rio Grande do Sul, Dept Oral Pathol, Porto Alegre, RS - Brazil
[2] Univ Nove Julho, Dept Oral Pathol, Sao Paulo - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Cancer Chemotherapy and Pharmacology; v. 71, n. 2, p. 293-299, FEB 2013.
Web of Science Citations: 20
Abstract

The aim of the present study was to evaluate the effect of topical chamomile and corticosteroid treatment on the profile of tissue cytokines (IL-1 beta and TNF-alpha) in 5-fluorouracil-induced oral mucositis in hamsters. Thirty-six hamsters were randomly separated into three groups (12 animals each): Group I-without treatment (control); Group II-treatment with chamomile (Ad-Muc(A (R))); and Group III-treatment with corticosteroid (betamethasone elixir- Celestone(A (R))). The animals received an intraperitoneal injection of 5-fluorouracil on Days 0 and 2. On Days 3 and 4, the buccal mucosa was scratched and therapy was initiated on Day 5. Three animals from each group were killed on Days 0, 5, 10, and 14 and the buccal mucosa was removed. The streptavidin-biotin complex method was used to delineate the in situ distribution, localization, and semiquantitative analysis of IL-1 beta and TNF-alpha. Data from the semiquantitative analysis of immunohistochemical staining were comparatively analyzed using the Kruskal-Wallis test, followed by Dunn's multiple comparisons test. The distribution and localization of IL-1 beta and TNF-alpha immunolabeling were similar. These proteins exhibited a diffuse pattern distributed throughout the connective tissue. The epithelium and adipose tissue were negative for both proteins. The semiquantitative analysis revealed that immunolabeling of IL-1 beta and TNF-alpha increased in all groups with the development of mucositis. On Day 10 (period of peak mucositis), the group treated with chamomile had lower scores for both pro-inflammatory cytokines. Treatment with topical chamomile reduced the tissue levels of IL-1 beta and TNF-alpha, thereby demonstrating anti-inflammatory action in oral mucositis in hamsters. (AU)