Fibronectin induces MMP2 expression in human prostate cancer cells

Author(s)

Moroz, Andrei ^{[1, 2]} ; Delella, Flavia K. ^[1] ; Lacorte, Livia M. ^[1] ; Deffune, Elenice ^{[2, 3]} ; Felisbino, Sergio L. ^[1]

Affiliation

^[1] Univ Estadual Paulista, UNESP, Dept Morphol, Inst Biosci, BR-18618970 Botucatu, SP - Brazil

^[2] Univ Estadual Paulista, UNESP, Botucatu Med Sch, Ctr Blood Transfus, Cell Engn Lab, BR-18618970 Botucatu, SP - Brazil

^[3] Univ Estadual Paulista, UNESP, Dept Urol, Botucatu Med Sch, BR-18618970 Botucatu, SP - Brazil

• Document type: Journal article
• Source: Biochemical and Biophysical Research Communications; v. 430, n. 4, p. 1319-1321, JAN 25 2013.
• Web of Science Citations: 34

Abstract

High-grade prostate cancers express high levels of matrix metalloproteinases (MMPs), major enzymes involved in tumor invasion and metastasis. However, the tumor cell lines commonly employed for prostate cancer research express only small amounts of MMPs when cultivated as monolayer cultures, in common culture media. The present study was conducted to ascertain whether culture conditions that include fibronectin can alter MMP2 and MMP9 expression by the human prostatic epithelial cell lines RWPE-1, LNCaP and PC-3. These cells were individually seeded at 2 x 10(4) cells/cm(2), cultivated until they reached 80% confluence, and then exposed for 4 h to fibronectin, after which the conditioned medium was analyzed by gelatin zymography. Untreated cells were given common medium. Only RWPE-1 cells express detectable amounts of MMP9 when cultivated in common medium, whereas the addition of fibronectin induced high expression levels of pro and active forms of MMP2 in all tested cell lines. Our findings demonstrate that normal and tumor prostate cell lines express MMP2 activity when in contact with extracellular matrix components or blood plasma proteins such as fibronectin. Future studies of transcriptomes and proteomes in prostate cancer research using these cell lines should not neglect these important conclusions. (C) 2012 Elsevier Inc. All rights reserved. (AU)

• FAPESP's process: 10/16671-3 - Finasteride effects on normal and tumoral prostatic cells: micro-RNA expression, cellular viability and cellular invasivity analysis
• Grantee: Sérgio Luis Felisbino
• Support Opportunities: Regular Research Grants