Overexpression of ANXA1 in Penile Carcinomas Positive for High-Risk HPVs

Author(s):Show less -

Calmon, Marilia Freitas ^[1] ; de Oliveira Mota, Manlio Tasso ^[1] ; Babeto, Erica ^[1] ; Candido, Natalia Maria ^[1] ; Girol, Ana Paula ^[1] ; Mendiburu, Carlos Fabian ^[2] ; Bonilha, Jane Lopes ^[3] ; Duarte Silvestre, Rodrigo Vellasco ^[4] ; Rosa, Bruno Miziara ^[2] ; Thome, Jorge Alberto ^[2] ; Americo Medeiros, Gustavo Hernandez ^[5] ; Soares, Fernando Augusto ^[5] ; Guimaraes, Gustavo Cardoso ^[5] ; Ferraz de Arruda, Jose Germano ^[3] ; Oliani, Sonia Maria ^[1] ; Villa, Luisa Lina ^{[6, 7]} ; Vassallo, Jose ^[5] ; Rahal, Paula ^[1]

Affiliation

^[1] Sao Paulo State Univ, Sao Paulo - Brazil

^[2] Inst Anat Pathol & Cytopathol, Sao Paulo - Brazil

^[3] Coll Med Rio Preto, Sao Paulo - Brazil

^[4] Univ Hosp Joao de Berros Barreto, Belem, Para - Brazil

^[5] Hosp AC Camargo Fund Antonio Prudente, Sao Paulo - Brazil

^[6] Univ Sao Paulo, Sch Med, Dept Radiol & Basic Oncol, Sao Paulo - Brazil

^[7] Coll Med Sci Santa Casa Sao Paulo, Sao Paulo - Brazil

• Document type: Journal article
• Source: PLoS One; v. 8, n. 1 JAN 14 2013.
• Web of Science Citations: 13

Abstract

The incidence of penile cancer varies between populations but is rare in developed nations. Penile cancer is associated with a number of established risk factors and associated diseases including phimosis with chronic inflammation, human papillomavirus (HPV) infection, poor hygiene and smoking. The objective of this study was to identify genes related to this type of cancer. The detection of HPV was analyzed in 47 penile squamous cell carcinoma samples. HPV DNA was detected in 48.9% of penile squamous cell carcinoma cases. High-risk HPV were present in 42.5% of cases and low-risk HPV were detected in 10.6% of penile squamous cell carcinomas. The RaSH approach identified differential expression of Annexin A1 (ANXA1), p16, RPL6, PBEF1 and KIAA1033 in high-risk HPV positive penile carcinoma; ANXA1 and p16 were overexpressed in penile squamous cells positive for high-risk HPVs compared to normal penile samples by qPCR. ANXA1 and p16 proteins were significantly more expressed in the cells from high-risk HPV-positive penile carcinoma as compared to HPV-negative tumors (p<0.0001) independently of the subtype of the carcinoma. Overexpression of ANXA1 might be mediated by HPV E6 in penile squamous cell carcinoma of patients with high-risk HPVs, suggesting that this gene plays an important role in penile cancer. (AU)

• FAPESP's process: 09/52088-3 - Cancer of the penis, a real Brazilian problem: from morphology to molecular etiopathogenesis
• Grantee: José Vassallo
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 09/11203-4 - Functional analysis of genes involved in carcinogenesis and development of penile cancer
• Grantee: Marilia de Freitas Calmon
• Support Opportunities: Scholarships in Brazil - Post-Doctoral