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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Analysis of ripening-related gene expression in papaya using an Arabidopsis-based microarray

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Author(s):
Fabi, Joao Paulo [1] ; Seymour, Graham B. [2] ; Graham, Neil S. [2] ; Broadley, Martin R. [2] ; May, Sean T. [2] ; Lajolo, Franco Maria [1, 3] ; Cordenunsi, Beatriz Rosana [1, 3] ; Oliveira do Nascimento, Joao Roberto [1, 3]
Total Authors: 8
Affiliation:
[1] Univ Sao Paulo, FCF, Dept Food Sci & Expt Nutr, Sao Paulo - Brazil
[2] Univ Nottingham, Sch Biosci, Plant & Crop Sci Div, Loughborough LE12 5RD, Leics - England
[3] Univ Sao Paulo, NAPAN Food & Nutr Res Ctr, Sao Paulo - Brazil
Total Affiliations: 3
Document type: Journal article
Source: BMC PLANT BIOLOGY; v. 12, DEC 21 2012.
Web of Science Citations: 22
Abstract

Background: Papaya (Carica papaya L.) is a commercially important crop that produces climacteric fruits with a soft and sweet pulp that contain a wide range of health promoting phytochemicals. Despite its importance, little is known about transcriptional modifications during papaya fruit ripening and their control. In this study we report the analysis of ripe papaya transcriptome by using a cross-species (XSpecies) microarray technique based on the phylogenetic proximity between papaya and Arabidopsis thaliana. Results: Papaya transcriptome analyses resulted in the identification of 414 ripening-related genes with some having their expression validated by qPCR. The transcription profile was compared with that from ripening tomato and grape. There were many similarities between papaya and tomato especially with respect to the expression of genes encoding proteins involved in primary metabolism, regulation of transcription, biotic and abiotic stress and cell wall metabolism. XSpecies microarray data indicated that transcription factors (TFs) of the MADS-box, NAC and AP2/ERF gene families were involved in the control of papaya ripening and revealed that cell wall-related gene expression in papaya had similarities to the expression profiles seen in Arabidopsis during hypocotyl development. Conclusion: The cross-species array experiment identified a ripening-related set of genes in papaya allowing the comparison of transcription control between papaya and other fruit bearing taxa during the ripening process. (AU)

FAPESP's process: 02/12452-9 - Carbohydrate metabolism during fruit ripening: a functional genomics approach
Grantee:Franco Maria Lajolo
Support Opportunities: Research Projects - Thematic Grants