| Full text | |
| Author(s): |
Total Authors: 4
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| Affiliation: | [1] Univ Cruzeiro Sul, Postgrad Program, CBS, BR-03342000 Sao Paulo - Brazil
Total Affiliations: 1
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| Document type: | Journal article |
| Source: | International Immunopharmacology; v. 14, n. 4, p. 690-697, DEC 2012. |
| Web of Science Citations: | 6 |
| Abstract | |
During normal B- and T-cell life, processes including activation, proliferation, signaling pathways and apoptosis are markedly dependent on ROS generation. However, these cells can also suffer the effect of oxidant overproduction. Thus, the purpose of the present study was to examine the possible pro-oxidant effects of MGO/high glucose and antioxidant effects of astaxanthin associated with vitamin Con some oxidative and antioxidant parameters of human lymphocytes in vitro. Lymphocytes from healthy subjects were treated with 20 mM of glucose and 30 mu M MGO followed or not by the addition of the antioxidants astaxanthin (2 mu M) and vitamin C (100 mu M) for up to 24 h. We examined superoxide dismutase (SOD), catalase, glutathione peroxidase, glutathione reductase, and glucose-6-phosphate dehydrogenase (G6PDH) activities, GSH/GSSG ratio and total thiol and carbonyl content. Oxidative parameters included superoxide anion, hydrogen peroxide and nitric oxide production. The association of astaxanthin and vitamin C proved to be a powerful antioxidant in human lymphocytes as showed by the marked reduction in superoxide anion, and hydrogen peroxide production as well as increased GSH content, GSH/GSSG ratio, GPx and GR activities. The antioxidant association showed to be more potent than their individual application. High glucose and methylglyoxal did not promote oxidative stress in human lymphocytes, since neither the oxidative parameters nor the antioxidant defense system was altered. According to these results, new therapies with the association of astaxanthin and vitamin C may be helpful to improve the immune function of patients with exacerbated production of ROS. (C) 2012 Elsevier B.V. All rights reserved. (AU) | |
| FAPESP's process: | 09/14382-7 - Effect of methylglyoxal and glucose on the function of human leukocytes: possible protective role of astaxantin and vitamin C |
| Grantee: | Rosemari Otton |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 10/03304-2 - Effect of the treatment with glucose and methylglyoxal on the function of human lymphocytes: possible protective role of astaxanthin and vitamin C |
| Grantee: | Anaysa Paola Bolin |
| Support Opportunities: | Scholarships in Brazil - Master |