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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Biostimulatory effects of low-level laser therapy on epithelial cells and gingival fibroblasts treated with zoledronic acid

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Author(s):
Basso, F. G. [1] ; Pansani, T. N. [2] ; Turrioni, A. P. S. [2] ; Kurachi, C. [3] ; Bagnato, V. S. [3] ; Hebling, J. [2] ; de Souza Costa, C. A. [2]
Total Authors: 7
Affiliation:
[1] Univ Estadual Campinas, Piracicaba Sch Dent, BR-13414903 Piracicaba, SP - Brazil
[2] Univ Estadual Paulista, Araraquara Sch Dent, BR-14801903 Araraquara, SP - Brazil
[3] Univ Sao Paulo, Inst Phys, Sao Carlos, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Laser Physics; v. 23, n. 5 MAY 2013.
Web of Science Citations: 4
Abstract

Low-level laser therapy (LLLT) has been considered as an adjuvant treatment for bisphosphonate-related osteonecrosis, presenting positive clinical outcomes. However, there are no data regarding the effect of LLLT on oral tissue cells exposed to bisphosphonates. This study aimed to evaluate the effects of LLLT on epithelial cells and gingival fibroblasts exposed to a nitrogen-containing bisphosphonate-zoledronic acid (ZA). Cells were seeded in wells of 24-well plates, incubated for 48 h and then exposed to ZA at 5 mu M for an additional 48 h. LLLT was performed with a diode laser prototype-LaserTABLE (InGaAsP-780 nm +/- 3 nm, 25 mW), at selected energy doses of 0.5, 1.5, 3, 5, and 7 J cm(-2) in three irradiation sessions, every 24 h. Cell metabolism, total protein production, gene expression of vascular endothelial growth factor (VEGF) and collagen type I (Col-I), and cell morphology were evaluated 24 h after the last irradiation. Data were statistically analyzed by Kruskal-Wallis and Mann-Whitney tests at 5% significance. Selected LLLT parameters increased the functions of epithelial cells and gingival fibroblasts treated with ZA. Gene expression of VEGF and Col-I was also increased. Specific parameters of LLLT biostimulated fibroblasts and epithelial cells treated with ZA. Analysis of these in vitro data may explain the positive in vivo effects of LLLT applied to osteonecrosis lesions. (AU)

FAPESP's process: 09/54722-1 - In vitro evaluation of the effects of low level laser therapy on cultured osteoblasts, fibroblasts and keratonicytes submitted to zoledronic acid therapy
Grantee:Carlos Alberto de Souza Costa
Support Opportunities: Regular Research Grants