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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Topical application of the lectin Artin M accelerates wound healing in rat oral mucosa by enhancing TGF-beta and VEGF production

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Author(s):
Kim, Yeon J. [1] ; Carvalho, Fernanda C. [2] ; Souza, Joao A. C. [1] ; Goncalves, Pedro C. G. [1] ; Nogueira, Andressa V. B. [1] ; Spolidorio, Luis C. [1] ; Roque-Barreira, Maria C. [2] ; Cirelli, Joni A. [1]
Total Authors: 8
Affiliation:
[1] UNESP Univ Estadual Paulista, Dept Diag & Surg, Div Periodontol, Sch Dent, BR-14801903 Sao Paulo - Brazil
[2] Univ Sao Paulo, Dept Cellular & Mol Biol, Sch Med Ribeirao Preto, Sao Paulo - Brazil
Total Affiliations: 2
Document type: Journal article
Source: WOUND REPAIR AND REGENERATION; v. 21, n. 3, p. 456-463, MAY-JUN 2013.
Web of Science Citations: 13
Abstract

The lectin Artin M has been shown to accelerate the wound-healing process. The aims of this study were to evaluate the effects of Artin M on wound healing in the palatal mucosa of rats and to investigate the effects of Artin M on transforming growth factor beta (TGF-) and vascular endothelial growth factor (VEGF) secretion by rat gingival fibroblasts. A surgical wound was created on the palatal mucosa of 72 rats divided into three groups according to treatment: CControl (nontreated), AArtin M gel, and VVehicle. Eight animals per group were sacrificed at 3, 5, and 7 days postsurgery for histology, immunohistochemistry and determination of the levels of cytokines, and growth factors. Gingival fibroblasts were incubated with 2.5g/mL of Artin M for 24, 48, and 72 hours. The expression of VEGF and TGF- was determined by enzyme-linked immunosorbent assay. Histologically, at day 7, the Artin M group showed earlier reepithelialization, milder inflammatory infiltration, and increased collagen fiber formation, resulting in faster maturation of granular tissue than in the other groups (p<0.05). Artin Minduced cell proliferation in vivo and promoted a greater expression of TGF- and VEGF in both experiments (p<0.05). Artin M was effective in healing oral mucosa wounds in rats and was associated with increased TGF- and VEGF release, cell proliferation, reepithelialization, and collagen deposition and arrangement of fibers. (AU)

FAPESP's process: 06/60642-2 - Lectins: biological effects and pharmaceutical applications
Grantee:Maria Cristina Roque Antunes Barreira
Support type: Research Projects - Thematic Grants
FAPESP's process: 09/16432-1 - Evaluation of Artin M on wound healing in palatal mucosa: an in vitro and in vivo study
Grantee:Joni Augusto Cirelli
Support type: Regular Research Grants
FAPESP's process: 09/16955-4 - Evaluation of Artin M gel on wound healing in rat palatal mucosa
Grantee:Pedro César Garcia Gonçalves
Support type: Scholarships in Brazil - Scientific Initiation