Evaluation of the effects of the lectin Artin M in the expression of tissue repair...
| Full text | |
| Author(s): |
Kim, Yeon J.
[1]
;
Carvalho, Fernanda C.
[2]
;
Souza, Joao A. C.
[1]
;
Goncalves, Pedro C. G.
[1]
;
Nogueira, Andressa V. B.
[1]
;
Spolidorio, Luis C.
[1]
;
Roque-Barreira, Maria C.
[2]
;
Cirelli, Joni A.
[1]
Total Authors: 8
|
| Affiliation: | [1] UNESP Univ Estadual Paulista, Dept Diag & Surg, Div Periodontol, Sch Dent, BR-14801903 Sao Paulo - Brazil
[2] Univ Sao Paulo, Dept Cellular & Mol Biol, Sch Med Ribeirao Preto, Sao Paulo - Brazil
Total Affiliations: 2
|
| Document type: | Journal article |
| Source: | WOUND REPAIR AND REGENERATION; v. 21, n. 3, p. 456-463, MAY-JUN 2013. |
| Web of Science Citations: | 13 |
| Abstract | |
The lectin Artin M has been shown to accelerate the wound-healing process. The aims of this study were to evaluate the effects of Artin M on wound healing in the palatal mucosa of rats and to investigate the effects of Artin M on transforming growth factor beta (TGF-) and vascular endothelial growth factor (VEGF) secretion by rat gingival fibroblasts. A surgical wound was created on the palatal mucosa of 72 rats divided into three groups according to treatment: CControl (nontreated), AArtin M gel, and VVehicle. Eight animals per group were sacrificed at 3, 5, and 7 days postsurgery for histology, immunohistochemistry and determination of the levels of cytokines, and growth factors. Gingival fibroblasts were incubated with 2.5g/mL of Artin M for 24, 48, and 72 hours. The expression of VEGF and TGF- was determined by enzyme-linked immunosorbent assay. Histologically, at day 7, the Artin M group showed earlier reepithelialization, milder inflammatory infiltration, and increased collagen fiber formation, resulting in faster maturation of granular tissue than in the other groups (p<0.05). Artin Minduced cell proliferation in vivo and promoted a greater expression of TGF- and VEGF in both experiments (p<0.05). Artin M was effective in healing oral mucosa wounds in rats and was associated with increased TGF- and VEGF release, cell proliferation, reepithelialization, and collagen deposition and arrangement of fibers. (AU) | |
| FAPESP's process: | 06/60642-2 - Lectins: biological effects and pharmaceutical applications |
| Grantee: | Maria Cristina Roque Antunes Barreira |
| Support type: | Research Projects - Thematic Grants |
| FAPESP's process: | 09/16432-1 - Evaluation of Artin M on wound healing in palatal mucosa: an in vitro and in vivo study |
| Grantee: | Joni Augusto Cirelli |
| Support type: | Regular Research Grants |
| FAPESP's process: | 09/16955-4 - Evaluation of Artin M gel on wound healing in rat palatal mucosa |
| Grantee: | Pedro César Garcia Gonçalves |
| Support type: | Scholarships in Brazil - Scientific Initiation |