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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

The CATS (FAM64A) protein is a substrate of the Kinase Interacting Stathmin (KIS)

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Archangelo, Leticia Froehlich [1] ; Greif, Philipp A. [2, 3] ; Maucuer, Alexandre [4] ; Manceau, Valerie [4] ; Koneru, Naresh [2, 3] ; Bigarella, Carolina L. [1] ; Niemann, Fernanda [1] ; dos Santos, Marcos Tadeu [5] ; Kobarg, Joerg [5] ; Bohlander, Stefan K. [2, 3, 6] ; Olalla Saad, Sara Teresinha [1]
Total Authors: 11
[1] State Univ Campinas UNICAMP, Hematol & Hemotherapy Ctr, BR-13083878 Campinas, SP - Brazil
[2] Univ Munich, Dept Med 3, D-81377 Munich - Germany
[3] Helmholtz Zentrum Munchen, Clin Cooperat Grp Leukemia, Natl Res Ctr Environm Hlth, D-81377 Munich - Germany
[4] Inst Fer Moulin, INSERM, U839, F-75005 Paris - France
[5] Natl Ctr Res Energy & Mat CNPEM, Natl Lab Biosci LNBio, BR-13083970 Campinas, SP - Brazil
[6] Univ Marburg, Ctr Human Genet, Marburg - Germany
Total Affiliations: 6
Document type: Journal article
Web of Science Citations: 8

The CATS protein (also known as FAM64A and RCS1) was first identified as a novel CALM (PICALM) interactor that influences the subcellular localization of the leukemogenic fusion protein CALM/AF10. CATS is highly expressed in cancer cell lines in a cell cycle dependent manner and is induced by mitogens. CATS is considered a marker for proliferation, known to control the metaphase-to-anaphase transition during the cell division. Using CATS as a bait in a yeast two-hybrid screen we identified the Kinase Interacting Stathmin (MS or UHMK1) protein as a CATS interacting partner. The interaction between CATS and KIS was confirmed by GST pull-down, co-immunopreciptation and co-localization experiments. Using kinase assay we showed that CATS is a substrate of KIS and mapped the phosphorylation site to CATS serine 131 (S131). Protein expression analysis revealed that KIS levels changed in a cell cycle-dependent manner and in the opposite direction to CATS levels. In a reporter gene assay KIS was able to enhance the transcriptional repressor activity of CATS, independent of CATS phophorylation at S131. Moreover, we showed that CATS and KIS antagonize the transactivation capacity of CALM/AF10.In summary, our results show that CATS interacts with and is a substrate for KIS, suggesting that KIS regulates CATS function. (c) 2013 Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 07/54870-5 - Functional characterization of the protein and its role in cellular proliferation and leukemogenesis
Grantee:Sara Teresinha Olalla Saad
Support type: Regular Research Grants
FAPESP's process: 07/08019-1 - Functional characterization of the CATS protein and its role in cellular proliferation and leukemogenesis
Grantee:Leticia Fröhlich Archangelo
Support type: Scholarships in Brazil - Post-Doctorate