Antimicrobial activity of calcium hydroxide and chlorhexidine on intratubular Candida albicans

Author(s):Show less -

Rezende Delgado, Ronan Jacques ^[1] ; Gasparoto, Thais Helena ^[1] ; Sipert, Carla Renata ^[1] ; Pinheiro, Claudia Ramos ^[1] ; de Moraes, Ivaldo Gomes ^[2] ; Garcia, Roberto Brandao ^[2] ; Hungaro Duarte, Marco Antonio ^[2] ; Bramante, Clovis Monteiro ^[2] ; Torres, Sergio Aparecido ^[1] ; Garlet, Gustavo Pompermaier ^[1] ; Campanelli, Ana Paula ^[1] ; Bernardineli, Norberti ^[2]

Affiliation

^[1] Univ Sao Paulo, Bauru Sch Dent, Dept Biol Sci, BR-17012901 Bauru - Brazil

^[2] Univ Sao Paulo, Bauru Sch Dent, Dept Operat Dent Endodont & Dent Mat, BR-17012901 Bauru - Brazil

• Document type: Journal article
• Source: International Journal of Oral Science; v. 5, n. 1, p. 32-36, MAR 2013.
• Web of Science Citations: 10

Abstract

This study investigated the efficacy of calcium hydroxide and chlorhexidine gel for the elimination of intratubular Candida albicans (C. albicans). Human single-rooted teeth contaminated with C. albicans were treated with calcium hydroxide, 2% chlorhexidine gel, calcium hydroxide plus 2% chlorhexidine gel, or saline (0.9% sodium chloride) as a positive control. The samples obtained at depths of 0-100 and 100-200 mu m from the root canal system were analyzed for C. albicans load by counting the number of colony forming units and for the percentage of viable C. albicans using fluorescence microscopy. First, the antimicrobial activity of calcium hydroxide and the 2% chlorhexidine gel was evaluated by counting the number of colony forming units. After 14 days of intracanal medication, there was a significant decrease in the number of C. albicans colony forming units at a depth of 0-100 pm with chlorhexidine treatment either with or without calcium hydroxide compared with the calcium hydroxide only treatment. However, there were no differences in the number of colony forming units at the 100-200 mu m depth for any of the medications investigated. C. albicans viability was also evaluated by vital staining techniques and fluorescence microscopy analysis. Antifungal activity against C. albicans significantly increased at both depths in the chlorhexidine groups with and without calcium hydroxide compared with the groups treated with calcium hydroxide only. Treatments with only chlorhexidine or chlorhexidine in combination with calcium hydroxide were effective for elimination of C. albicans. International Journal of Oral Science (2013) 5, 32-36; doi:10.1038/ijos.2013.12; published online 29 March 2013 (AU)

• FAPESP's process: 07/00306-1 - Expression of procollagen type I, MIP-1alpha and SDF-1alpha by human gingival fibroblasts and dental pulp stimulated by Enterococcus faecalis lipoteichoic acid and lipopolysaccharide from Escherichia coli and Porphyromonas gingivalis
• Grantee: Carla Renata Sipert
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 09/14127-7 - The role of inflammasomes in chemically induced tumor
• Grantee: Thaís Helena Gasparoto
• Support Opportunities: Scholarships in Brazil - Post-Doctoral