Microbiological profile of donor corneas stored for tectonic transplantation purposes in rabbits

Author(s)

Kobashigawa, K. K. ^[1] ; Martins, B. C. ^{[2, 1]} ; Massoli, M. C. B. ^[1] ; Ishizawa, T. H. ^[1] ; Schocken-Iturrino, R. P. ^[1] ; Brooks, D. E. ^[2] ; Laus, J. L. ^[1]

Affiliation

^[1] UNESP, Fac Ciencias Agr & Vet, Jaboticabal, SP - Brazil

^[2] Univ Florida, Gainesville, FL - USA

• Document type: Journal article
• Source: Arquivo Brasileiro de Medicina Veterinária e Zootecnia; v. 65, n. 1, p. 61-66, FEB 2013.
• Web of Science Citations: 0

Abstract

This study aimed to evaluate the microbiota of donor rabbit corneas stored for tectonic transplantation purposes. Swabs from both corneas of 20 rabbits were carefully collected and submitted to microorganism isolation and identification. After this first swab collection, rabbits were euthanized for reasons other than this project and the eyes were enucleated. The corneas were collected and stored to compose the cornea tissue bank. Corneas were stored in a 0.3% tobramycin solution at -20ºC. After 30 days, the corneas were thawed at room temperature and removed from the antibiotic. New swabs were obtained from the corneas and submitted to microorganism isolation and identification. Gram positive organisms were predominant in the rabbit corneal flora before storage and the Staphylococcus sp. was the most common microorganism isolated from those samples. No growth was observed on the samples collected after storage. The methods used for collection and storage of the corneas were efficient to constitute a sterile donor corneal tissue bank. (AU)

• FAPESP's process: 09/14384-0 - Microbiological study of corneal bank dedicated to corneal transplantation in rabbits (Oryctolagus cuniculus - Lilljeborgi, 1874)
• Grantee: Karina Kamachi Kobashigawa
• Support Opportunities: Scholarships in Brazil - Scientific Initiation