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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Characterization of polymorphic microsatellite markers for the Neotropical leaf-frog Phyllomedusa burmeisteri and cross-species amplification

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Author(s):
Brunes, T. O. [1, 2] ; van de Vliet, M. S. [3] ; Lopes, S. [1] ; Alexandrino, J. [4] ; Haddad, C. F. B. [2] ; Sequeira, F. [1]
Total Authors: 6
Affiliation:
[1] Univ Porto, Ctr Invest Biodiversidade & Recursos Genet, CIBIO UP, Vairao - Portugal
[2] Univ Estadual Paulista, Inst Biociencias, Dept Zool, Rio Claro, SP - Brazil
[3] Univ Algarve, CIMAR Lab Associado, Ctr Invest Marinha & Ambiental, Fac Ciencias Mar & Ambiente, Faro - Portugal
[4] Univ Fed Sao Paulo, Dept Ciencias Biol, Diadema, SP - Brazil
Total Affiliations: 4
Document type: Journal article
Source: Genetics and Molecular Research; v. 12, n. 1, p. 242-247, 2013.
Web of Science Citations: 1
Abstract

Twelve polymorphic tetranucleotide microsatellite loci were isolated and characterized for the leaf-frog Phyllomedusa burmeisteri, an endemic species of the Brazilian Atlantic forest. These loci were screened in 25 individuals from two populations of the Minas Gerais State (Carangola and Juiz de Fora). The number of alleles per locus ranged from 3 to 16 (mean = 8). Observed and expected heterozygosities ranged from 0.25 to 0.92 and 0.56 to 0.92, respectively. Evidence for both the presence of null alleles and Hardy-Weinberg equilibrium deviations were found in loci Phybu4, Phybu17, and Phybu21. Genotypic disequilibrium for each pair of loci across populations was not significant. Cross-species amplification was successful for 11 of the 12 developed loci for the sister-species, P. bahiana. These microsatellites will be important for future fine-scale population structure analyses. (AU)

FAPESP's process: 05/52727-5 - Biogeography, phylogeography and diversification patterns of anuran species widespread in the Brazilian Atlantic forest
Grantee:João Miguel de Barros Alexandrino
Support type: Research Grants - Young Investigators Grants
FAPESP's process: 08/50928-1 - Speciation of frogs in high-altitude environments
Grantee:Célio Fernando Baptista Haddad
Support type: Research Projects - Thematic Grants