Acidulated Phosphate Fluoride Application Changes the Protein Composition of Human Acquired Enamel Pellicle

Author(s)

Masson, N. ^{[1, 2]} ; Domingues, R. R. ^[2] ; Cury, J. A. ^[1] ; Paes Leme, A. F. ^[2]

Affiliation

^[1] Univ Estadual Campinas, Piracicaba Dent Sch, UNICAMP, Piracicaba - Brazil

^[2] CNPEM, Brazilian Biosci Natl Lab, LNBio, Mass Spectrometry Lab, BR-13083970 Campinas, SP - Brazil

• Document type: Journal article
• Source: Caries Research; v. 47, n. 3, p. 251-258, 2013.
• Web of Science Citations: 6

Abstract

We evaluated, by proteomic analysis, whether the chemical changes provoked on enamel by acidulated phosphate fluoride (APF) application alter the protein composition of acquired enamel pellicle. Enamel slabs, pretreated with distilled water (negative control), phosphoric acid (active control) or APF solution, were immersed in human saliva for pellicle formation. The adsorbed proteins were extracted and analyzed by liquid chromatography-mass spectrometry/mass spectrometry. Fifty-six proteins were identified, 12 exclusive to APF and 11 to phosphoric acid. APF decreased the concentration of histatin-1, but increased the concentration of S100-A9, which is confirmed by immunoblotting. The findings suggest that APF application changes the acquired enamel pellicle composition. Copyright (C) 2013 S. Karger AG, Basel (AU)

• FAPESP's process: 09/12368-7 - Proteomic analysis of acquired enamel/dentine pellicle treated with fluoride
• Grantee: Nádia Masson
• Support type: Scholarships in Brazil - Master
• FAPESP's process: 10/19278-0 - Study of regulation of ADAMs in oral cancer
• Grantee: Adriana Franco Paes Leme
• Support type: Research Grants - Young Investigators Grants