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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Acidulated Phosphate Fluoride Application Changes the Protein Composition of Human Acquired Enamel Pellicle

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Masson, N. [1, 2] ; Domingues, R. R. [2] ; Cury, J. A. [1] ; Paes Leme, A. F. [2]
Total Authors: 4
[1] Univ Estadual Campinas, Piracicaba Dent Sch, UNICAMP, Piracicaba - Brazil
[2] CNPEM, Brazilian Biosci Natl Lab, LNBio, Mass Spectrometry Lab, BR-13083970 Campinas, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Caries Research; v. 47, n. 3, p. 251-258, 2013.
Web of Science Citations: 6

We evaluated, by proteomic analysis, whether the chemical changes provoked on enamel by acidulated phosphate fluoride (APF) application alter the protein composition of acquired enamel pellicle. Enamel slabs, pretreated with distilled water (negative control), phosphoric acid (active control) or APF solution, were immersed in human saliva for pellicle formation. The adsorbed proteins were extracted and analyzed by liquid chromatography-mass spectrometry/mass spectrometry. Fifty-six proteins were identified, 12 exclusive to APF and 11 to phosphoric acid. APF decreased the concentration of histatin-1, but increased the concentration of S100-A9, which is confirmed by immunoblotting. The findings suggest that APF application changes the acquired enamel pellicle composition. Copyright (C) 2013 S. Karger AG, Basel (AU)

FAPESP's process: 09/12368-7 - Proteomic analysis of acquired enamel/dentine pellicle treated with fluoride
Grantee:Nádia Masson
Support type: Scholarships in Brazil - Master
FAPESP's process: 10/19278-0 - Study of regulation of ADAMs in oral cancer
Grantee:Adriana Franco Paes Leme
Support type: Research Grants - Young Investigators Grants