On the relationship between the solubility of proteins and the osmotic second virial coefficient

Author(s)

Franco, L. F. M. ^[1] ; Pessoa Filho, P. de A. ^[1]

Affiliation

^[1] Univ Sao Paulo, Sch Engn, Dept Chem Engn, Bioproc Engn Grp, BR-05424970 Sao Paulo - Brazil

• Document type: Journal article
• Source: Brazilian Journal of Chemical Engineering; v. 30, n. 1, p. 95-104, JAN-MAR 2013.
• Web of Science Citations: 4

Abstract

A relationship between the osmotic second virial coefficient of proteins in aqueous salt solutions, the solubility of proteins in these solutions and the salt concentration is presented. The model developed considers that the solid-liquid equilibrium is established with neutral protein molecules and that the relationship between the protein solubility and the salt concentration follows Cohn's equation. The validity of the model is restricted to the salting-out region of the phase diagrams, which is the situation of greater practical importance. The resulting equations were successfully applied to systems containing lysozyme and ovalbumin. (AU)

• FAPESP's process: 09/06575-0 - Thermodynamic modeling of solid-liquid equilibrium in protein aqueous systems: effect of pH
• Grantee: Luís Fernando Mercier Franco
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 08/11232-1 - Study on the influence of co-solvents on thermodynamic properties of proteins in solution
• Grantee: Pedro de Alcântara Pessôa Filho
• Support Opportunities: Regular Research Grants