In Vitro Cytotoxicity of White MTA, MTA Fillapex® and Portland Cement on Human Periodontal Ligament Fibroblasts

Author(s)

Patrícia Yoshino ^[1] ; Celso Kenji Nishiyama ^[2] ; Karin Cristina da Silva Modena ^[3] ; Carlos Ferreira Santos ^[4] ; Carla Renata Sipert ^[5]

Affiliation

^[1] University of São Paulo (HRAC-USP). Hospital for Rehabilitation of Craniofacial Anomalies. Endodontics Division - Brasil

^[2] University of São Paulo (HRAC-USP). Hospital for Rehabilitation of Craniofacial Anomalies. Endodontics Division - Brasil

^[3] USP - University of São Paulo. Bauru Dental School. Endodontics and Dental Materials - Brasil

^[4] University of São Paulo (HRAC-USP). Hospital for Rehabilitation of Craniofacial Anomalies. Endodontics Division - Brasil

^[5] University of São Paulo (HRAC-USP). Hospital for Rehabilitation of Craniofacial Anomalies. Endodontics Division - Brasil

• Document type: Journal article
• Source: Brazilian Dental Journal; v. 24, n. 2, p. 111-116, 2013-04-00.

Abstract

The aim of this study was to compare the in vitro cytotoxicity of white mineral trioxide aggregate (MTA), MTA Fillapex® and Portland cement (PC) on human cultured periodontal ligament fibroblasts. Periodontal ligament fibroblast culture was established and the cells were used for cytotoxic tests after the fourth passage. Cell density was set at 1.25 X10 4 cells/well in 96-well plates. Endodontic material extracts were prepared by placing sealer/cement specimens (5X3mm) in 1mL of culture medium for 72 h. The extracts were then serially two-fold diluted and inserted into the cell-seeded wells for 24, 48 and 72 h. MTT assay was employed for analysis of cell viability. Cell supernatants were tested for nitric oxide using the Griess reagent system. MTA presented cytotoxic effect in undiluted extracts at 24 and 72 h. MTA Fillapex® presented the highest cytotoxic levels with important cell viability reduction for pure extracts and at ½ and ¼ dilutions. In this study, PC did not induce alterations in fibroblast viability. Nitric oxide was detected in extract-treated cell supernatants and also in the extracts only, suggesting presence of nitrite in the soluble content of the tested materials. In the present study, MTA Fillapex displayed the highest cytotoxic effect on periodontal ligament fibroblasts followed by white MTA and PC. (AU)

• FAPESP's process: 07/00306-1 - Expression of procollagen type I, MIP-1alpha and SDF-1alpha by human gingival fibroblasts and dental pulp stimulated by Enterococcus faecalis lipoteichoic acid and lipopolysaccharide from Escherichia coli and Porphyromonas gingivalis
• Grantee: Carla Renata Sipert
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 05/60167-0 - Expression of procollagen type 1, MIP-1± alpha and SDF-1 alpha by human fibroblasts stimulated pulp lipoteichoic acid from Streptococcus mutans
• Grantee: Carlos Ferreira dos Santos
• Support Opportunities: Regular Research Grants