Effect of laser phototherapy in the prevention and treatment of chemo-induced mucositis in hamsters

Author(s)

Talita Christine Camilo Lopez ^[1] ; Manoela Domingues Martins ^[2] ; Vanessa Christina Santos Pavesi ^[3] ; Leila Soares Ferreira ^[4] ; Sandra Kalil Bussadori ^[3] ; Maria Stella Moreira ^[4] ; Marcia Martins Marques ^[1]

Affiliation

^[1] Univ Sao Paulo, Dept Restorat Dent, Sch Dent, Sao Paulo - Brazil

^[2] Univ Fed Rio Grande do Sul UFRGS, Sch Dent, Dept Oral Pathol, Porto Alegre, RS - Brazil

^[3] Univ Nove Julho Uninove, Sch Dent, Postgrad Program Rehabil Sci, Sao Paulo - Brazil

^[4] Univ Ibirapuera Unib, Sch Dent, Postgrad Program Dent, Sao Paulo - Brazil

• Document type: Journal article
• Source: Brazilian Oral Research; v. 27, n. 4, p. 342-348, 2013-06-11.

Abstract

The aim of this study was to investigate the effect of laser phototherapy (LPT) in the prevention and/or treatment of oral mucositis induced by 5-fluorouracil (5-FU; Eurofarma, São Paulo, Brazil) in hamsters. Ninety-six hamsters were divided into four groups (n = 24): Control (no treatment); Preventive [LPT from day (D) D-5 to D+5]; Therapeutic (LPT from D+5 to D+15); and Combined (preventive plus therapeutic LPT from D-5 to D+15). The animals received an intraperitoneal injection of 5-FU on Days 0 and 2. The pouch mucosa was scratched on Days 3 and 4. The irradiation parameters were: indium-gallium-aluminum-phosphide (InGaAlP) diode laser (MM Optics, São Carlos, Brazil) (660 nm), beam area of 0.036 cm2, 40 mW, 1.11 W/cm2, 6.6 J/cm2, power density applied daily of 39.6 J/cm2, in punctual mode (six points and six seconds per point) and contact mode, one application per day. The animals were sacrificed on Days 0, 5, 10 and 15 (n = 6) and weighed, and the pouch mucosa was removed for histopathological analysis. Clinical and corresponding histological scores were compared using ANOVA and Tukey's test (p ≤0.05). Similar weight losses ranging from 5% to 10% occurred in all groups. The therapeutic group had significantly lower clinical and histological scores than the other groups at Day 10. This study showed that positive effects on oral mucositis management were obtained only when LPT was applied in the therapeutic protocol (from D+5 to D+15 after chemotherapy). (AU)

• FAPESP's process: 09/10254-4 - Pre-clinical study of low power laser phototherapy (LPT) in the prevention and rehabilitation of chemo-induced mucosites in hamsters
• Grantee: Talita Christine Camilo Lopez
• Support Opportunities: Scholarships in Brazil - Scientific Initiation