| Full text | |
| Author(s): |
Willian Marinho Dourado Coelho
[1]
;
Jancarlo Ferreira Gomes
[2, 3]
;
Alessandro Francisco Talamini do Amarante
[4]
;
Katia Denise Saraiva Bresciani
[1]
;
Giuliano Lumina
[4]
;
Sumie Koshino-Shimizu
[5]
;
Denise Pereira Leme
[6]
;
Alexandre Xavier Falcão
[8]
Total Authors: 8
|
| Affiliation: | [1] Univ Estadual Paulista UNESP, BR-16050680 Aracatuba, SP - Brazil
[2] Univ Estadual Campinas UNICAMP, Inst Biol, Campinas, SP - Brazil
[3] Univ Estadual Campinas UNICAMP, Inst Computacao, Campinas, SP - Brazil
[4] Univ Estadual Paulista UNESP, Inst Biociencias, Botucatu, SP - Brazil
[5] Univ Sao Paulo, Inst Trop Med, Sao Paulo - Brazil
[6] Fundacao Municipal Ensino Super Braganca Paulista, Fac Med Vet, Braganca Paulista, SP - Brazil
[7] Universidade Estadual de Campinas – UNICAMP. Instituto de Biologia e Instituto de Computação - Brasil
Total Affiliations: 7
|
| Document type: | Journal article |
| Source: | Revista Brasileira de Parasitologia Veterinária; v. 22, n. 1, p. 1-5, 2013-03-00. |
| Abstract | |
In this study, we aimed to introduce a new technique calledTF-Test Modified∕Dog for the diagnosis of gastrointestinal parasites in dogs. Fecal samples from 106 dogs were processed by the technique TF-Test Modified∕Dog and the techniques of centrifugation-flotation in zinc sulfate, simple-flotation by saturated solution of sodium chloride, direct microscopy exam and TF-Test Conventional. Sensitivity was higher in the TF-Test Modified∕Dog (98.41%), followed by flotation in saturated zinc sulfate (77.78%), TF-Test Conventional (73.02%), flotation by saturated sodium chloride (55.55%), and direct microscopy exam (30.16%). The diagnostic efficiency varied from 58.49% to 99.06%, with the highest value also obtained by the new proposed technique. Efficiency level of 99.06% with kappa index 0.979 (almost perfect) was obtained with the TF-Test Modified∕Dog. These results represent significant statistical gains (P < 0.05) of 20.63% in sensitivity and 12.27% in efficiency over the best among the other techniques – flotation by saturated zinc sulfate, whose kappa index was 0.738, much lower than that of the TF-Test Modified∕Dog. All techniques presented 100% specificity. In this sense, the high sensitivity of theTF-Test Modified∕Dog makes it suitable for epidemiological surveys of gastrointestinal parasitosis in dogs, zoonoses control and preventive surveillance programs. (AU) | |