Pluripotent stem cell transcription factors during human odontogenesis

Author(s)

da Cunha, Juliana Malta ^[1] ; da Costa-Neves, Adriana ^[2] ; Kerkis, Irina ^[3] ; Pereira da Silva, Marcelo Cavenaghi ^[1]

Affiliation

^[1] Univ Fed Sao Paulo, Dept Morfol & Genet, Sao Paulo - Brazil

^[2] Inst Butantan, Lab Biol Celular, Sao Paulo - Brazil

^[3] Inst Butantan, Genet Lab, Sao Paulo - Brazil

• Document type: Journal article
• Source: Cell and Tissue Research; v. 353, n. 3, p. 435-441, SEP 2013.
• Web of Science Citations: 11

Abstract

Stem cells are capable of generating various cell lines and can be obtained from adult or embryonic tissues for clinical therapies. Stem cells from deciduous dental pulp are among those that are easily obtainable from adult tissues and have been widely studied because of their ability to differentiate into a variety of cell lines in the presence of various chemical mediators. We have analyze the expression of several proteins related to the differentiation and proliferative potential of cell populations that compose the tooth germ of human fetuses. We evaluate 20 human fetuses of both genders. After being paraffin-embedded, cap and bell stages of tooth germ development were subjected to immunohistochemistry for the following markers: Oct-4, Nanog, Stat-3 and Sox-2. The studied antibodies showed nuclear or cytoplasmic immunnostaining within various anatomical structures and with various degrees of expression, indicating the action of these proteins during tooth development. We conclude that the interrelationship between these transcription factors is complex and associated with self-renewal and cell differentiation. Our results suggest that the expression of Oct-4, Nanog, Sox-2 and Stat-3 are related to differentiation in ameloblasts and odontoblasts. (AU)

• FAPESP's process: 10/08085-7 - Expression of markers related to cell differentiation potential in human fetus dental germ
• Grantee: Marcelo Cavenaghi Pereira da Silva
• Support Opportunities: Regular Research Grants