Microorganisms in cryopreserved semen and culture media used in the in vitro production (IVP) of bovine embryos identified by matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS)

Author(s):Show less -

Zampieri, Davila ^[1] ; Santos, Vanessa G. ^[1] ; Braga, Patricia A. C. ^[1] ; Ferreira, Christina R. ^[1] ; Ballottin, Daniela ^[2] ; Tasic, Ljubica ^[2] ; Basso, Andrea C. ^[3] ; Sanches, Bruno V. ^[3] ; Pontes, Jose H. F. ^[3] ; da Silva, Barbara Pereira ^[4] ; Garboggini, Fabiana Fantinatti ^[4] ; Eberlin, Marcos N. ^[1] ; Tata, Alessandra ^[1]

Affiliation

^[1] Univ Estadual Campinas, Inst Chem, ThoMSon Mass Spectrometry Lab, Campinas, SP - Brazil

^[2] Univ Estadual Campinas, Inst Chem, Biol Chem Lab, Campinas, SP - Brazil

^[3] InVitro Brasil Ltda, Mogi Mirim, SP - Brazil

^[4] Univ Estadual Campinas, Chem Biol & Agr Pluridisciplinary Res Ctr CPQBA, Microbial Resources Div, Campinas, SP - Brazil

• Document type: Journal article
• Source: Theriogenology; v. 80, n. 4, p. 337-345, SEP 1 2013.
• Web of Science Citations: 3

Abstract

Commercial cattle breeders produce their own herd offspring for the dairy and beef market using artificial insemination. The procedure involves sanitary risks associated with the collection and commercialization of the germplasm, and the in vitro production and transfer of the bovine embryos must be monitored by strict health surveillance. To avoid the spreading of infectious diseases, one must rely on using controlled and monitored germplasm, media, and reagents that are guaranteed free of pathogens. In this article, we investigated the use of a new mass spectrometric approach for fast and accurate identification of bacteria and fungi in bovine semen and in culture media employed in the embryo in vitro production process. The microorganisms isolated from samples obtained in a commercial bovine embryo IVP setting were identified in a few minutes by their conserved peptide/protein profile, obtained applying matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS), matched against a commercial database. The successful microorganisms MS identification has been confirmed by DNA amplification and sequencing. Therefore, the MS technique seems to offer a powerful tool for rapid and accurate microorganism identification in semen and culture media samples. (C) 2013 Elsevier Inc. All rights reserved. (AU)

• FAPESP's process: 11/06191-7 - MALDI-TOF and ESI-MS fingerprinting for bacteria identification in culture media and semen as a tool for sanitary excelence in bovine embryo in vitro production
• Grantee: Alessandra Tata
• Support Opportunities: Scholarships in Brazil - Post-Doctoral