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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Microbial diversity of hydrogen-producing bacteria in batch reactors fed with cellulose using leachate as inoculum

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Author(s):
Ratti, Regiane Priscila [1] ; Botta, Livia Silva [1] ; Sakamoto, Isabel Kimiko [1] ; Amancio Varesche, Maria Bernadete [1]
Total Authors: 4
Affiliation:
[1] Univ Sao Paulo, Dept Hydraul & Sanitat, Sch Engn Sao Carlos, BR-13563120 Sao Carlos, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: INTERNATIONAL JOURNAL OF HYDROGEN ENERGY; v. 38, n. 23, p. 9707-9717, AUG 6 2013.
Web of Science Citations: 22
Abstract

Hydrogen production using cellulosic residues offers the possibility of waste minimization with renewable energy recovery. In the present study, heat-treated biomass purified from leachate was used as inoculum in batch reactors for hydrogen production fed with different concentrations of cellulose (2.5, 5.0 and 10 g/L), in the presence and absence of exogenous cellulase. The heat-treated biomass did not degrade cellulose and hydrogen production was not detected in the absence of cellulase. In reactors with cellulase, the hydrogen yields were 1.2, 0.6 and 2.3 mol H-2/mol of hydrolyzed cellulose with substrate degradation of 41.4, 28.4 and 44.7% for 2.5, 5.0 and 10 g/L cellulose, respectively. Hydrogen production potentials (P) varied from 19.9 to 125.9 mmol H-2 and maximum hydrogen production rates (R-m) were among 0.8-2.3 mmol H-2/h. The reactor containing 10 g/L of cellulose presented the highest P and R-m among the conditions tested. The main acid produced in reactors were butyric acid, followed by acetic, isobutyric and propionic acids. Bacteria similar to Clostridium sp. (98-99%) were identified in the reactors with cellulase. The heat-treated leachate can be used as an inoculum source for hydrogen production from hydrolyzed cellulose. Copyright (C) 2013, Hydrogen Energy Publications, LLC. Published by Elsevier Ltd. All rights reserved. (AU)

FAPESP's process: 10/01237-6 - Hydrolisis of sugar cane bagasse for hydrogen production in thermophilic condition
Grantee:Regiane Priscila Ratti Sartori
Support Opportunities: Scholarships in Brazil - Post-Doctoral