| Full text | |
| Author(s): |
Villabona-Arenas, Christian Julian
[1]
;
Mondini, Adriano
[2]
;
Bosch, Irene
[3]
;
Schimitt, Diane
[4]
;
Calzavara-Silva, Carlos E.
[5]
;
Zanotto, Paolo M. de A.
[1]
;
Nogueira, Mauricio L.
[6]
Total Authors: 7
|
| Affiliation: | [1] Univ Sao Paulo, Inst Ciencias Biomed, Dept Microbiol, LEMB, BR-05508 Sao Paulo - Brazil
[2] Univ Estadual Paulista Julio de Mesquita Filho Ar, Fac Ciencias Farmaceut, Dept Ciencias Biol, Lab Saude Publ, Sao Paulo - Brazil
[3] MIT, Div Hlth Sci & Technol, Cambridge, MA 02139 - USA
[4] Tufts Univ, Dept Infect Dis & Global Hlth, Cummings Sch Vet Med, North Grafton, MA - USA
[5] Fundacao Oswaldo Cruz Fiocruz, Ctr Pesquisas Rene Rachou CPqRR, LICM, Belo Horizonte, MG - Brazil
[6] Fac Med Sao Jose do Rio Preto, Lab Pesquisas Virol, Sao Jose Do Rio Preto, SP - Brazil
Total Affiliations: 6
|
| Document type: | Journal article |
| Source: | PLoS One; v. 8, n. 5 MAY 7 2013. |
| Web of Science Citations: | 7 |
| Abstract | |
Global dengue virus spread in tropical and sub-tropical regions has become a major international public health concern. It is evident that DENV genetic diversity plays a significant role in the immunopathology of the disease and that the identification of polymorphisms associated with adaptive responses is important for vaccine development. The investigation of naturally occurring genomic variants may play an important role in the comprehension of different adaptive strategies used by these mutants to evade the human immune system. In order to elucidate this role we sequenced the complete polyprotein-coding region of thirty-three DENV-3 isolates to characterize variants circulating under high endemicity in the city of Sao Jose de Rio Preto, Brazil, during the onset of the 2006-07 epidemic. By inferring the evolutionary history on a local-scale and estimating rates of synonymous (dS) and nonsynonimous (dN) substitutions, we have documented at least two different introductions of DENV-3 into the city and detected 10 polymorphic codon sites under significant positive selection (dN/dS > 1) and 8 under significant purifying selection (dN/dS < 1). We found several polymorphic amino acid coding sites in the envelope (15), NS1 (17), NS2A ( 11), and NS5 (24) genes, which suggests that these genes may be experiencing relatively recent adaptive changes. Furthermore, some polymorphisms correlated with changes in the immunogenicity of several epitopes. Our study highlights the existence of significant and informative DENV variability at the spatio-temporal scale of an urban outbreak. (AU) | |
| FAPESP's process: | 11/17071-2 - Phylogeography of urban outbreaks of dengue virus in Jundiaí and Guarujá in the State of São Paulo |
| Grantee: | Christian Julián Villabona Arenas |
| Support Opportunities: | Scholarships in Brazil - Doctorate (Direct) |
| FAPESP's process: | 12/11733-6 - Biological and molecular characterization of dengue isolates, serotypes 1 and 2, in São José do Rio Preto city, SP, detected during 2006 to 2014 seasons |
| Grantee: | Maurício Lacerda Nogueira |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 10/19059-7 - Phylogeography of urban outbreaks of dengue virus in Jundiaí and Guarujá in the State of São Paulo |
| Grantee: | Paolo Marinho de Andrade Zanotto |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 11/10458-9 - Dengue surveillance: is the detection of infected mosquitoes a sentinel event for dengue infections? |
| Grantee: | Maurício Lacerda Nogueira |
| Support Opportunities: | Regular Research Grants |