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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Short Report: Evaluation of Chemical Preparation on Insect Wing Shape for Geometric Morphometrics

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Lorenz, Camila [1, 2] ; Suesdek, Lincoln [2]
Total Authors: 2
[1] Inst Butantan, Dept Parasitol, Sao Paulo - Brazil
[2] Inst Butantan, Parasitol Lab, Sao Paulo - Brazil
Total Affiliations: 2
Document type: Journal article
Source: American Journal of Tropical Medicine and Hygiene; v. 89, n. 5, p. 928-931, NOV 2013.
Web of Science Citations: 8

Geometric morphometrics is an approach that has been increasingly applied in studies with insects. A limiting factor of this technique is that some mosquitoes have wings with dark spots or many scales, which jeopardizes the visualization of landmarks for morphometric analysis. Recently, in some studies, chemically treatment (staining) of the wings was used to improve the viewing of landmarks. In this study, we evaluated whether this method causes deformation of the wing veins and tested whether it facilitates the visualization of the most problematic landmarks. In addition, we tested whether mechanical removal of the scales was sufficient for this purpose. The results showed that the physical and chemical treatments are equally effective in improving visualization of the landmarks. The chemical method did not cause deformation of the wing. Thus, some of these treatments should be performed before beginning geometric morphometric analysis to avoid erroneous landmark digitizing. (AU)

FAPESP's process: 13/05521-9 - Characterization of macroevolutionary patterns in Culicidae (Diptera) using geometric morphometrics, genetic sequencing and mass spectrometry
Grantee:Camila Lorenz
Support type: Scholarships in Brazil - Doctorate