Characterization of macroevolutionary patterns in Culicidae (Diptera) using geometric morphometrics, genetic sequencing and mass spectrometry

• Grant number: 13/05521-9
• Support Opportunities: Scholarships in Brazil - Doctorate
• Effective date (Start): June 01, 2013
• Effective date (End): May 31, 2017
• Field of knowledge: Biological Sciences - Parasitology - Entomology and Malacology of Parasites and Vectors
• Principal Investigator: Lincoln Suesdek Rocha
• Grantee: Camila Lorenz
• Host Institution: Instituto Butantan. Secretaria da Saúde (São Paulo - Estado). São Paulo , SP, Brazil
• Associated research grant: 09/53637-0 - Anopheles-Plasmodium interaction: a multidisciplinary approach, AP.TEM
• Associated scholarship(s): 15/12784-1 - A mitochondrial genome phylogeny of Culicidae, BE.EP.DR

Abstract

Culicidae are popularly known as mosquitoes and comprise a monophyletic group within the order Diptera. They are distributed throughout tropical and temperate regions, and currently 3525 species are recognized. The traditional classification is based on morphological characters and despite their medical importance and long history of research, the taxonomy of the group remains incomplete and the classification system is not entirely natural. There are significant gaps in knowledge of the taxonomy of Culicidae, because it is a large group with many species. The aim of this work is to develop a hypothesis with phylogenetic resolution to the level of genera in Culicidae based on the traditional characters of genetic variability and the newly aggregated characters "protein profile" evidenced by mass spectrometry (MALDI-TOF). Sequences of various genes of the major taxa be obtained from GenBank and analyzed from phylogenetic point of view. Data of protein profiles evidenced by MALDI-TOF also be added in phylogenetic analyzes, by constructing supermatrix and (or) supertrees. Characters of geometry's wing will be employed additionally as taxonomic markers, especially in species of difficult identification, and used to construct a phenogram. Like other cases from the literature, the combination of different methods may be useful to understand the evolutionary history of the family Culicidae in a holistic mean. (AU)

Scientific publications

(11)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

AGUIAR, BRENO S.; LORENZ, CAMILA; VIRGINIO, FLAVIA; SUESDEK, LINCOLN; CHIARAVALLOTI-NETO, FRANCISCO. Potential risks of Zika and chikungunya outbreaks in Brazil: A modeling study. INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES, v. 70, p. 20-29, MAY 2018. (13/05521-9, 14/27172-9)

LORENZ, CAMILA; ALVES, JOAO M. P.; FOSTER, PETER G.; SUESDEK, LINCOLN; SALLUM, MARIA ANICE M.. Phylogeny and temporal diversification of mosquitoes (Diptera: Culicidae) with an emphasis on the Neotropical fauna. Systematic Entomology, v. 46, n. 4, p. 798-811, OCT 2021. (15/12784-1, 13/05521-9, 13/14622-3)

VIRGINIO, FLAVIA; DOMINGUES, VICTOR; GOMES DA SILVA, LETICIA CECILIA; ANDRADE, LUCAS; BRAGHETTO, KELLY ROSA; SUESDEK, LINCOLN. WingBank: A Wing Image Database of Mosquitoes. FRONTIERS IN ECOLOGY AND EVOLUTION, v. 9, APR 16 2021. (11/18962-8, 06/05164-8, 10/15039-1, 06/02622-5, 10/14479-8, 13/05521-9, 07/01665-5)

LORENZ, CAMILA; FERRAUDO, ANTONIO SERGIO; SUESDEK, LINCOLN. Artificial Neural Network applied as a methodology of mosquito species identification. Acta Tropica, v. 152, p. 165-169, DEC 2015. (13/05521-9)

LORENZ, CAMILA; VIRGINIO, FLAVIA; AGUIAR, BRENO S.; SUESDEK, LINCOLN; CHIARAVALLOTI-NETO, FRANCISCO. Spatial and temporal epidemiology of malaria in extra-Amazonian regions of Brazil. Malaria Journal, v. 14, OCT 15 2015. (13/05521-9)

LORENZ, CAMILA; PATANE, JOSE S. L.; SUESDEK, LINCOLN. Morphogenetic characterisation, date of divergence, and evolutionary relationships of malaria vectors Anopheles cruzii and Anopheles homunculus. INFECTION GENETICS AND EVOLUTION, v. 35, p. 144-152, OCT 2015. (13/05521-9)

LORENZ, CAMILA; SUESDEK, LINCOLN. Short Report: Evaluation of Chemical Preparation on Insect Wing Shape for Geometric Morphometrics. American Journal of Tropical Medicine and Hygiene, v. 89, n. 5, p. 928-931, NOV 2013. (13/05521-9)

LORENZ, CAMILA; PEREIRA ALVES, JOAO MARCELO; FOSTER, PETER GORDON; MUREB SALLUM, MARIA ANICE; SUESDEK, LINCOLN. First record of translocation in Culicidae (Diptera) mitogenomes: evidence from the tribe Sabethini. BMC Genomics, v. 20, n. 1, SEP 27 2019. (13/05521-9, 15/12784-1)

LORENZ, CAMILA; SUESDEK, LINCOLN. The use of wing shape for characterising macroevolution in mosquitoes (Diptera: Culicidae). INFECTION GENETICS AND EVOLUTION, v. 77, JAN 2020. (17/10297-1, 13/05521-9)

LORENZ, CAMILA; ALMEIDA, FABIO; ALMEIDA-LOPES, FERNANDA; LOUISE, CAROLINE; PEREIRA, STELLA N.; PETERSEN, VIVIAN; VIDAL, PALOMA O.; VIRGINIO, FLAVIA; SUESDEK, LINCOLN. Geometric morphometrics in mosquitoes: What has been measured?. INFECTION GENETICS AND EVOLUTION, v. 54, p. 205-215, OCT 2017. (13/26014-8, 13/26017-7, 10/15039-1, 06/02622-5, 14/27172-9, 12/17717-2, 13/05521-9)

LORENZ, CAMILA; AZEVEDO, THIAGO S.; VIRGINIO, FLAVIA; AGUIAR, BRENO S.; CHIARAVALLOTI-NETO, FRANCISCO; SUESDEK, LINCOLN. Impact of environmental factors on neglected emerging arboviral diseases. PLoS Neglected Tropical Diseases, v. 11, n. 9, SEP 2017. (13/05521-9)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

LORENZ, Camila. Use of morphometric techniques, MALDI-TOF spectrometry and genetic sequencing for classification and phylogeny of Culicidae (Diptera).. 2017. Doctoral Thesis - Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) São Paulo.