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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Phylogenetic and Chemical Diversity of a Hybrid-IsoprenoidProducing Streptomycete Lineage

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Author(s):
Gallagher, Kelley A. [1] ; Rauscher, Kristin [1] ; Ioca, Laura Pavan [1] ; Jensen, Paul R. [1]
Total Authors: 4
Affiliation:
[1] Univ Calif San Diego, Scripps Inst Oceanog, Ctr Marine Biotechnol & Biomed, La Jolla, CA 92093 - USA
Total Affiliations: 1
Document type: Journal article
Source: Applied and Environmental Microbiology; v. 79, n. 22, p. 6894-6902, NOV 2013.
Web of Science Citations: 10
Abstract

Streptomyces species dedicate a large portion of their genomes to secondary metabolite biosynthesis. A diverse and largely marine-derived lineage within this genus has been designated MAR4 and identified as a prolific source of hybrid isoprenoid (HI) secondary metabolites. These terpenoid-containing compounds are common in nature but rarely observed as bacterial secondary metabolites. To assess the phylogenetic diversity of the MAR4 lineage, complementary culture-based and culture-independent techniques were applied to marine sediment samples collected off the Channel Islands, CA. The results, including those from an analysis of publically available sequence data and strains isolated as part of prior studies, placed 40 new strains in the MAR4 clade, of which 32 originated from marine sources. When combined with sequences cloned from environmental DNA, 28 MAR4 operational taxonomic units (0.01% genetic distance) were identified. Of these, 82% consisted exclusively of either cloned sequences or cultured strains, supporting the complementarity of these two approaches. Chemical analyses of diverse MAR4 strains revealed the production of five different HI structure classes. All 21 MAR4 strains tested produced at least one HI class, with most strains producing from two to four classes. The two major clades within the MAR4 lineage displayed distinct patterns in the structural classes and the number and amount of HIs produced, suggesting a relationship between taxonomy and secondary metabolite production. The production of HI secondary metabolites appears to be a phenotypic trait of the MAR4 lineage, which represents an emerging model with which to study the ecology and evolution of HI biosynthesis. (AU)

FAPESP's process: 11/08064-2 - Investigation of the secondary metabolism of two fungal strains from the marine environment
Grantee:Laura Pavan Ióca
Support Opportunities: Scholarships in Brazil - Scientific Initiation