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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Methods for obtaining reliable and reproducible results in studies of Candida biofilms formed in vitro

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Author(s):
Costa, Anna C. B. P. [1, 2] ; Pereira, Cristiane A. [1] ; Freire, Fernanda [1] ; Junqueira, Juliana C. [1] ; Jorge, Antonio O. C. [1]
Total Authors: 5
Affiliation:
[1] UNESP Univ Estadual Paulista, Inst Sci & Technol, Dept Biosci & Oral Diag, Sao Jose Dos Campos - Brazil
[2] Hans Knoell Inst, Dept Microbial Pathogen Mech, D-07745 Jena - Germany
Total Affiliations: 2
Document type: Review article
Source: MYCOSES; v. 56, n. 6, p. 614-622, NOV 2013.
Web of Science Citations: 22
Abstract

Biofilm formation is one of the most important attributes for virulence in Candida species and contributes to increased resistance to antifungal drugs and host immune mechanisms. These features have led to the development of several methodologies to reproduce a sessile community in vitro that can be used to study the development of a biofilm, its interaction with other microorganisms and the environment, and its susceptibility to available antifungal agents and also to search for new therapy strategies. The purpose of this review is to describe the most commonly used methods to study Candida biofilms in vitro, to discuss the benefits and limitations of the different methods to induce biofilm formation, and to analyse the architecture, viability and growth kinetics of Candida biofilms. (AU)

FAPESP's process: 10/00879-4 - OPPORTUNISTIC MICROORGANISMS IN INJURIES denture stomatitis AND INFLUENCE OF PHOTODYNAMIC THERAPY IN VIRULENCE FACTORS OF Candida spp.
Grantee:Cristiane Aparecida Pereira Correia
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 10/18753-7 - Evaluation of photodynamic therapy on Candida albicans in vitro and in vivo
Grantee:Antonio Olavo Cardoso Jorge
Support Opportunities: Regular Research Grants
FAPESP's process: 11/21346-7 - GENES WITH UNKNOWN FUNCTION OF Candida albicans AS TO BIOFILM FORMATION, BIOLOGICAL CHARACTERISTICS AND HOST- PATHOGEN INTERACTION
Grantee:Anna Carolina Borges Pereira da Costa
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 12/09188-0 - Effects of the photodynamic therapy in the expression of the genes SAP5, PLB1 and LIP9 in Candida albicans
Grantee:Fernanda Freire
Support Opportunities: Scholarships in Brazil - Master