Comparison between cucurbiturils and beta-cyclodextrin interactions with cholesterol molecules present in Langmuir monolayers used as a biomembrane model

Author(s)

Tovani, Camila Bussola ^[1] ; Ventrici de Souza, Joao Francisco ^[1] ; Cavallini, Thiago de Souza ^[1] ; Demets, Gregoire Jean-Francois ^[1] ; Ito, Amando ^[2] ; Barioni, Marina Berardi ^[2] ; Pazin, Wallance Moreira ^[2] ; Darbello Zaniquelli, Maria Elisabete ^[1]

Affiliation

^[1] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Quim, BR-14040901 Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Fis, BR-14040901 Ribeirao Preto, SP - Brazil

• Document type: Journal article
• Source: COLLOIDS AND SURFACES B-BIOINTERFACES; v. 111, p. 398-406, NOV 1 2013.
• Web of Science Citations: 7

Abstract

Specific surface techniques can probe the interaction of cholesterol (Chol) with substances that are able to host and/or sequester this biomolecule, provided that the additives are properly assembled at the interface. Reports on inclusion complexes of Chol with beta-cyclodextrins exist in the literature. Here we compare the interaction of beta-cyclodextrin and cucurbiturils with Chol present in Langmuir phospholipid (dipalmitoylphosphatidylcholine, DPPC) monolayers, used as a biomembrane model. Cucurbiturils, CB{[}n], comprise macrocyclic host molecules consisting of n glycoluril units. Classic surface pressure curves, dilatational surface viscoelasticity measurements, and fluorescence emission spectra and images obtained by time-resolved fluorescence of the corresponding Langmuir-Blodgett films have shown that homologues with 5 and 6 glycoluril units, CB{[}5] and CB{[}6], do not form inclusion complexes. Higher-order homologues, such as CB{[}7], are likely to complex with Chol with changes in the minimum molecular areas recorded for DPPC/Chol monolayers, the fluorescence decay lifetimes, and the dilatational surface viscosities of the monolayers generated in the presence of these molecules. Moreover, we proof the removal of cholesterol from the biomimetic interface in the presence of CB{[}7] by means of fluorescence spectra from the subphase support of monolayers containing fluorescent-labeled Chol. (C) 2013 Elsevier B.V. All rights reserved. (AU)

• FAPESP's process: 11/00850-9 - Curcubiturils as cholesterol carrier in biomembrane model systems
• Grantee: Maria Elisabete Darbello Zaniquelli
• Support Opportunities: Regular Research Grants
• FAPESP's process: 09/54044-3 - Acquisition of a fluorescence correlation spectroscope for the measurement of fluorescence correlation and image capture by fluorescence lifetime
• Grantee: Amando Siuiti Ito
• Support Opportunities: Multi-user Equipment Program