Investigation of Cultivable Bacteria Isolated from Longstanding Retreatment-resistant Lesions of Teeth with Apical Periodontitis

Author(s)

Signoretti, Fernanda G. C. ^[1] ; Gomes, Brenda P. F. A. ^[1] ; Montagner, Francisco ^[2] ; Jacinto, Rogerio C. ^{[1, 3]}

Affiliation

^[1] Univ Estadual Campinas, Piracicaba Dent Sch, Endodont Div, Dept Restorat Dent, Piracicaba, SP - Brazil

^[2] Univ Fed Rio Grande do Sul, Endodont Div, Porto Alegre, RS - Brazil

^[3] Univ Fed Pelotas, Endodont Div, BR-96015560 Pelotas, RS - Brazil

• Document type: Journal article
• Source: JOURNAL OF ENDODONTICS; v. 39, n. 10, p. 1240-1244, OCT 2013.
• Web of Science Citations: 8

Abstract

Introduction: The objective of this research was to investigate the presence of viable bacteria in tissue samples from persistent apical lesions and to correlate the microbiological findings with the histopathological diagnosis of the lesion. Methods: Twenty persistent apical lesions associated with well-performed endodontic retreatment were collected. Tissue samples were processed through culture techniques including serial dilution, plating, aerobic and anaerobic incubation, and biochemical tests for microbial identification followed by histopathological diagnosis. Results: Cysts were more frequently diagnosed (13/20). Strict anaerobic species predominated in both cysts (80.4% of the species detected) and granulomas (65% of the species detected). Viable gram-positive bacteria were frequently recovered from apical lesions (cysts = 70.6%, granulomas = 84.4%). Gemella morbillorum and Propionibacterium acnes were the most frequently recovered species from cysts and granulomas, respectively. At least 1 gram-positive bacterial species was present in almost every sample (cysts = 12/13, granulomas = 7/7). No significant correlation was found between histologic findings and bacterial species. Conclusions: In conclusion, although cysts were more frequent than granulomas in cases of failure of endodontic retreatment, bacteria were isolated from both types of lesions, with a predominance of gram-positive species, suggesting that these species can survive outside the root canal and might be related to the persistence of the pathological process even after accurate endodontic retreatment. (AU)

• FAPESP's process: 11/09047-4 - Investigation of microbial diversity from primary endodontic infection by clonal analysis and 16S rRNA sequencing
• Grantee: Brenda Paula Figueiredo de Almeida Gomes
• Support Opportunities: Regular Research Grants