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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Dynamics of bone healing after osteotomy with piezosurgery or conventional drilling - histomorphometrical, immunohistochemical, and molecular analysis

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Author(s):
Esteves, Jonatas Caldeira [1, 2] ; Marcantonio, Jr., Elcio [3] ; de Souza Faloni, Ana Paula [4] ; Godoy Rocha, Fernanda Regina [1] ; Marcantonio, Rosemary Adriana [1] ; Wilk, Katarzyna [2] ; Intini, Giuseppe [2, 5]
Total Authors: 7
Affiliation:
[1] Univ Estadual Paulista, UNESP, Araraquara Dent Sch, Dept Diagnost & Sugery, Sao Paulo - Brazil
[2] Harvard Univ, Harvard Sch Dent Med, Dept Oral Med Infect & Immun, Boston, MA 02115 - USA
[3] UNESP, Univ Estadual Paulista, Araraquara Dent Sch, Dept Diag & Surg Periodont, Araraquara - Brazil
[4] Univ Ctr Araraquara, UNIARA, Implantol Post Graduat Course, Sao Paulo - Brazil
[5] Harvard Stem Cell Inst, Cambridge, MA - USA
Total Affiliations: 5
Document type: Journal article
Source: JOURNAL OF TRANSLATIONAL MEDICINE; v. 11, SEP 23 2013.
Web of Science Citations: 25
Abstract

Background: Piezosurgery is an osteotomy system used in medical and dental surgery. Many studies have proven clinical advantages of piezosurgery in terms of quality of cut, maneuverability, ease of use, and safety. However, few investigations have tested its superiority over the traditional osteotomy systems in terms of dynamics of bone healing. Therefore, the aim of this study was to evaluate the dynamics of bone healing after osteotomies with piezosurgery and to compare them with those associated to traditional bone drilling. Methods: One hundred and ten rats were divided into two groups with 55 animals each. The animals were anesthetized and the tibiae were surgically exposed to create defects 2 mm in diameter by using piezosurgery (Piezo group) and conventional drilling (Drill group). Animals were sacrificed at 3, 7, 14, 30 and 60 days post-surgery. Bone samples were collected and processed for histological, histomorphometrical, immunohistochemical, and molecular analysis. The histological analysis was performed at all time points (n = 8) whereas the histomorphometrical analysis was performed at 7, 14, 30 and 60 days post-surgery (n = 8). The immunolabeling was performed to detect Vascular Endothelial Growth Factor (VEGF), Caspase-3 (CAS-3), Osteoprotegerin (OPG), Receptor Activator of Nuclear Factor kappa-B Ligand (RANKL), and Osteocalcin (OC) at 3, 7, and 14 days (n = 3). For the molecular analysis, animals were sacrificed at 3, 7 and 14 days, total RNA was collected, and quantification of the expression of 21 genes related to BMP signaling, Wnt signaling, inflammation, osteogenenic and apoptotic pathways was performed by qRT-PCR (n = 5). Results: Histologically and histomorphometrically, bone healing was similar in both groups with the exception of a slightly higher amount of newly formed bone observed at 30 days after piezosurgery (p < 0.05). Immunohistochemical and qRT-PCR analyses didn't detect significant differences in expression of all the proteins and most of the genes tested. Conclusions: Based on the results of our study we conclude that in a rat tibial bone defect model the bone healing dynamics after piezosurgery are comparable to those observed with conventional drilling. (AU)

FAPESP's process: 10/16645-2 - Bone healing around dental implants placed in surgical alveolus prepared using drills, piezosurgery and Er,Cr:YSGG laser - radiographic, biomechanical, histomorphometric and immunohistochemical study in rats
Grantee:Elcio Marcantonio Junior
Support Opportunities: Regular Research Grants
FAPESP's process: 12/16764-7 - Exression of cytokines and gorwth factors in the bone repair of defects produced by burs and surgical ultrassound
Grantee:Jônatas Caldeira Esteves
Support Opportunities: Scholarships abroad - Research Internship - Doctorate
FAPESP's process: 10/07712-8 - Bone healing around dental implants placed in surgical alveolus prepared using drills, piezosurgery and Er,Cr:YSGG laser - Radiographic, biomechanical, histomorphometric and immunohistochemical study in rats.
Grantee:Jônatas Caldeira Esteves
Support Opportunities: Scholarships in Brazil - Doctorate