Adjunct effect of the antimicrobial photodynamic therapy to an association of non-surgical and surgical periodontal treatment in modulation of gene expression: A human study

Author(s)

Andrade, Patricia F. ^[1] ; Garlet, Gustavo P. ^[2] ; Silva, Joao S. ^[3] ; Fernandes, Patricia G. ^[1] ; Milanezi, Cristiane ^[3] ; Novaes, Jr., Arthur B. ^[1] ; Palioto, Daniela B. ^[1] ; Grisi, Marcio F. M. ^[1] ; Taba, Jr., Mario ^[1] ; Souza, Sergio L. S. ^[1]

Affiliation

^[1] Univ Sao Paulo, Sch Dent Ribeirao Preto, Dept Buccomaxillofacial Surg & Traumatol & Period, BR-14040904 Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Sch Dent Bauru, Dept Biol Sci, Bauru, SP - Brazil

^[3] Univ Sao Paulo, Sch Med Ribeirao Preto, Dept Immunol, BR-14040904 Ribeirao Preto, SP - Brazil

• Document type: Journal article
• Source: JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY; v. 126, p. 119-125, SEP 5 2013.
• Web of Science Citations: 6

Abstract

Background: This study has evaluated the effect of antimicrobial photodynamic therapy (aPDT) used in conjunction with non-surgical and surgical periodontal treatment (PT) in modulating gene expression during periodontal wound healing. Methods: Fifteen patients with chronic periodontitis, presenting bilaterally lower molars with class III furcation lesions and scheduled for extraction, were selected. In initial therapy, scaling and root planing (SRP) was performed in the Control Group (CG), while SRP + aPDT were performed in the Test Group (TG). 45 days later, flap surgery plus SRP, and flap surgery plus SRP + aPDT were performed in the CG and TG, respectively. At 21 days post-surgery, the newly formed granulation tissue was collected, and Real-time PCR evaluated the expression of the genes: tumor necrosis factor-alpha, interleukin-1 beta, interleukin-4, interleukin-10, matrix metalloproteinase-2 (MMP-2), tissue inhibitor of metalloproteinase-2 (TIMP-2), osteoprotegerin (OPG), receptor activator of nuclear factor-kappa B ligand (RANKL), type I collagen, alkaline phosphatase, osteopontin, osteocalcin, and bone sialoprotein. Results: There were statistically significant differences between the groups in relation to mRNA levels for MMP-2 (TG = 3.26 +/- 0.89; CG = 4.23 +/- 0.97; p = 0.01), TIMP-2/MMP-2 ratio (TG = 0.91 +/- 0.34; CG = 0.73 +/- 0.32; p = 0.04), OPG (TG = 0.84 +/- 0.45; CG = 0.30 +/- 0.26; p = 0.001), and OPG/RANKL ratio (TG = 0.60 +/- 0.86; CG = 0.23 +/- 0.16; p = 0.04), favoring the TG. Conclusion: The present data suggest that the aPDT associated to nonsurgical and surgical periodontal therapy may modulate the extracellular matrix and bone remodeling by up regulating the TIMP- 2/MMP-2 and OPG/RANKL mRNA ratio, but the clinical relevance needs to be evaluated in further studies. (c) 2013 Elsevier B.V. All rights reserved. (AU)

• FAPESP's process: 07/04916-9 - Evaluation of the photodynamic therapy on root decontamination and modulation of the immuno-inflammatory response and healing of the periodontal tissues: a randomized controlled clinical study in humans
• Grantee: Sérgio Luís Scombatti de Souza
• Support Opportunities: Regular Research Grants