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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Spatial distribution and population genetics of Leishmania infantum genotypes in Sao Paulo State, Brazil, employing multilocus microsatellite typing directly in dog infected tissues

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Motoie, Gabriela [1] ; Melim Ferreira, Gabriel Eduardo [2] ; Cupolillo, Elisa [2] ; Canavez, Flavio [3] ; Pereira-Chioccola, Vera Lucia [1]
Total Authors: 5
[1] Adolfo Lutz Inst, Lab Parasitol, BR-01246902 Sao Paulo - Brazil
[2] Fiocruz MS, Inst Oswaldo Cruz, Lab Pesquisa Leishmaniose, BR-21045900 Rio De Janeiro - Brazil
[3] Genoa Biotecnol SA, Sao Paulo - Brazil
Total Affiliations: 3
Document type: Journal article
Source: INFECTION GENETICS AND EVOLUTION; v. 18, p. 48-59, AUG 2013.
Web of Science Citations: 12

This study investigated the genetic characteristics of Leishmania infantum samples from Sao Paulo (SP) State, Brazil in order to collaborate with information about the possible origins of the parasites, as well as, the introduction and spread of visceral leishmaniasis in this Brazilian State. Multilocus microsatellite typing (MLMT) was performed using a set of 17 microsatellite markers. DNA was extracted from 250 samples collected from dogs diagnosed with visceral leishmaniasis and 112 (45%) were genotyped: 67 from the northwest region (NWSP), and 29 from the southeast region (SESP) of SP. The results were correlated with other 16 samples from Mato Grosso do Sul State (MS) (which borders NWSP). Although, a small portion of samples was genotyped, it was possible to genotype multiple lad using small amounts of Leishmania DNA extracted directly from dog tissues. Despite the fact that MLMT analysis defined 33 different genotypes, a low polymorphism was detected within the parasites studied with 10 polymorphic loci. There are two main genetic clusters circulating in SP with strong genetic differentiation, one (POP-A) is composed by samples from SESP and NWSP and presented a weak signal of geographical substructure. The other, belongs to the same cluster found in the state of MS (POP-B), which was the main one. The majority (93.75%) of MS parasite genotypes belonged to POP-B, with just one sample (6.25%) grouped in POP-A. POP-B also comprised 10.34% of SESP and 26.87% of NWSP samples. Besides one sample from MS, POP-A is composed by 73.13% of NWSP and 89.66% of SESP samples. The MLMT analysis supported the idea of canine visceral leishmaniasis being introduced in the Northwest region of SP State by the traffic of humans and dogs from MS. In the southeast region of SP occurred an introduction of a new L infantum genetic cluster. Probably the transmission was spread by traffic of infected dogs from other Brazilian regions, or by introduction of imported dogs from other countries. All these data together contributed to the detection of the genetic profile of L. infantum population in SP State. (C) 2013 Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 11/13939-8 - Study of a group of fungal and parasitic infections aiming the improvement of techniques of characterization and immune-molecular diagnosis
Grantee:Vera Lúcia Pereira Chioccola
Support type: Regular Research Grants