Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

A immunosensor for the diagnosis of canine distemper virus infection using SPR and EIS

Full text
Author(s):
Basso, Caroline Rodrigues [1] ; Tozato, Claudia de Camargo [2] ; Mendes Ribeiro, Marcela Cristina [2] ; Araujo Junior, Joao Pessoa [2] ; Pedrosa, Valber A. [1]
Total Authors: 5
Affiliation:
[1] UNESP, Inst Biosci, Dept Chem & Biochem, BR-18618000 Botucatu, SP - Brazil
[2] UNESP, Inst Biosci, Dept Microbiol & Immunol, BR-18618000 Botucatu, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: ANALYTICAL METHODS; v. 5, n. 19, p. 5089-5095, 2013.
Web of Science Citations: 6
Abstract

Canine distemper virus (CDV) is a viral disease that affects dogs and many other carnivores. Clinical diagnosis of CDV is difficult due to the broad spectrum of signs that may be confounded with other respiratory and enteric diseases of dogs. Laboratory analysis is required to diagnose suspected cases. In this study, surface plasmon resonance (SPR) and electrochemical impedance spectroscopy (EIS) methodologies were developed for the detection of canine distemper virus simultaneously. The assay exhibited high specificity, as all the negative controls were not mistakenly detected. The CDV concentration was determined from successive injections into the apparatus, with a linear range from 1.1 to 116.0 ng mL(-1). The system exhibited good reproducibility with 4.5% variation between runs after regeneration of the coated surface with a solution of 0.1 M glycine-HCL (pH 3.0). The capacitance and resistance values of the modified interface were calculated from EIS data using an equivalent circuit. It was possible to measure CDV in highly concentrated viruses with good specificity and reproducibility. (AU)

FAPESP's process: 12/15666-1 - Detection of canine distemper virus by different analytical methodologies
Grantee:Caroline Rodrigues Basso
Support Opportunities: Scholarships in Brazil - Master
FAPESP's process: 10/07478-5 - Chain reaction amplified biocatalytic responses in nanostructured plasmonic sensors
Grantee:Valber de Albuquerque Pedrosa
Support Opportunities: Research Grants - Young Investigators Grants