Analytical methods for copper, zinc and iron quantification in mammalian cells

Author(s)

Cerchiaro, Giselle ^[1] ; Manieri, Tania Maria ^[1] ; Bertuchi, Fernanda Rodrigues ^[1]

Affiliation

^[1] Fed Univ ABC UFABC, Ctr Nat Sci & Humanities, Santo Andre, SP - Brazil

• Document type: Review article
• Source: METALLOMICS; v. 5, n. 10, p. 1336-1345, 2013.
• Web of Science Citations: 34

Abstract

Highly complex analytical methods with different accuracies of measurement, reproducibilities and ease of analyses are currently being used to quantify metals in cellular media and tissue samples. In this review, the analytical methods commonly used for iron, copper and zinc quantification in mammalian cells are presented and discussed. Herein, we present a literature survey of the most commonly found concentrations of these metals in various mammalian cells in culture and tissues. The aim of this review is to help researchers in metallomic-related areas identify the method that best suits their needs for the accurate quantification of these metals in cells. This accuracy goes beyond simple knowledge of the limit of detection of each technique and needs to be evaluated through comparisons with similar previous studies. (AU)

• FAPESP's process: 12/01397-9 - Effects of metalloenzyme superoxide dismutase expression in the generation of reactive oxygen species in cultured mammalian cells and in motor neurons
• Grantee: Giselle Cerchiaro
• Support Opportunities: Regular Research Grants