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(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Molecular and Structural Evaluation of Dentin Caries-Like Lesions Produced by Different Artificial Models

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Author(s):
Larissa Ferreira Pacheco [1] ; Efani Caroline de Freitas Banzi [2] ; Eliana Rodrigues [3] ; Luis Eduardo Silva Soares [4] ; Fernanda Miori Pascon [5] ; Lourenco Correr-Sobrinho [6] ; Regina Maria Puppin-Rontani [7]
Total Authors: 7
Affiliation:
[1] UNICAMP - University of Campinas. Piracicaba School of Dentistry. Department of Pediatric Dentistry - Brasil
[2] UNICAMP - University of Campinas. Piracicaba School of Dentistry. Department of Pediatric Dentistry - Brasil
[3] UNICAMP - University of Campinas. Piracicaba School of Dentistry. Department of Pediatric Dentistry - Brasil
[4] IP&D, Laboratory of Biomedical Vibrational Spectroscopy. Research and Development Institute. Vale do Paraiba University; School of Dentistry - Brasil
[5] UNICAMP - University of Campinas. Piracicaba School of Dentistry. Department of Pediatric Dentistry - Brasil
[6] UNICAMP - University of Campinas. Piracicaba School of Dentistry. Dental Materials Division - Brasil
[7] UNICAMP - University of Campinas. Piracicaba School of Dentistry. Department of Pediatric Dentistry - Brasil
Total Affiliations: 7
Document type: Journal article
Source: Brazilian Dental Journal; v. 24, n. 6, p. 610-618, 2013-12-00.
Abstract

This study evaluated structural and molecular issues of dentin caries-like lesions produced by different artificial models (ACL) compared with natural caries lesions (NCL). One hundred twenty-four sound occlusal dentin blocks and 47 carious blocks were obtained and surface hardness was analyzed (SH1). They were assigned to groups according to ACL: GB: Biological; GC: Chemical; GIS: In situ; GNC: natural caries (control). Blocks from groups 1, 2 and 3 were submitted to caries lesion induction. NCL and ACL blocks were submitted to surface hardness (SH 2), FT-Raman and µEDXRF analysis. All blocks were longitudinally sectioned and one of the halves was submitted to cross-sectional hardness (CSH) and the other to SEM analysis. SH1 and SH2 data were submitted to t test (unpaired and paired, respectively), CSH and SEM data to two-way and one-way ANOVA respectively, and Tukey and t tests, respectively (p<0.05). Data from FT-Raman/µEDXRF were submitted to one-way ANOVA and Dunnett multiple-comparisons test (a=0.05). GB and GNC showed lowest SH2 values that were significantly different from GC and GIS. Regarding CSH, GB and GNC showed no significant difference between them. SEM showed similar caries lesion depth for GB and GNC, being significantly higher than for GC and GIS. µEDXRF showed similar values of calcium and phosphate for GB and GNC; GNC values were significantly different from GIS. No significant difference was found among the groups concerning phosphate, carbonate and CH bonds values. For collagen type I, GC values were significantly different compared to other groups. It may be concluded that caries-like lesions produced by GB were the closest model to NCL. (AU)

FAPESP's process: 08/02331-6 - Study of different dentin substrata in order to bond adhesives systems. Morphological, molecular and mechanical analysis
Grantee:Larissa Ferreira Pacheco
Support Opportunities: Scholarships in Brazil - Master