| Full text | |
| Author(s): |
Ferreira Queiroz, Marcos Marcal
[1]
;
Queiroz, Emerson Ferreira
[2]
;
Zeraik, Maria Luiza
[1]
;
Ebrahimi, Samad Nejad
[3]
;
Marcourt, Laurence
[2]
;
Cuendet, Muriel
[2]
;
Castro-Gamboa, Ian
[1]
;
Hamburger, Matthias
[3]
;
Bolzani, Vanderlan da Silva
[1]
;
Wolfender, Jean-Luc
[2]
Total Authors: 10
|
| Affiliation: | [1] Univ Estadual Paulista, UNESP, Nucleo Bioensaios Biossintese & Ecofisiol Prod Na, NuBBE, Inst Quim, Sao Paulo - Brazil
[2] Univ Lausanne, Univ Geneva, Sch Pharmaceut Sci, CH-1211 Geneva 4 - Switzerland
[3] Univ Basel, Div Pharmaceut Biol, CH-4056 Basel - Switzerland
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | Journal of Natural Products; v. 77, n. 3, p. 650-656, MAR 2014. |
| Web of Science Citations: | 20 |
| Abstract | |
The secondary metabolite content of Tetrapterys mucronata, a poorly studied plant that is used occasionally in Brazil for the preparation of a psychotropic plant decoction called ``Ayahuasca{''}, was determined to establish its chemical composition and to search for acetylcholinesterase (AChE) inhibitors. The ethanolic extract of the bark of T. mucronata exhibited in vitro AChE inhibition in a TLC bioautography assay. To localize the active compounds, biological profiling for AChE inhibition was performed using at-line HPLC-microfractionation in 96-well plates and subsequent AChE inhibition bioautography. The analytical HPLC-PDA conditions were transferred geometrically to a preparative medium-pressure liquid chromatography column using chromatographic calculations for the efficient isolation of the active compounds at the milligram scale. Twenty-two compounds were isolated, of which six are new natural products. The structures of the new compounds (9, 10, 16-18, and 20) were elucidated by spectroscopic data interpretation. Compounds 1, 5, 6, 9, and 10 inhibited AChE with IC50 values below 15 mu M. (AU) | |
| FAPESP's process: | 10/09780-0 - mass spectrometry and nuclear magnetic resonance in metabolomic assessment & identification of prototypes of AChE inhibitors in Banisteriopsis variabilis (B. Gates) - Malpighiaceae |
| Grantee: | Marcos Marçal Ferreira Queiroz |
| Support Opportunities: | Scholarships in Brazil - Doctorate |