Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Nanotopography Drives Stem Cell Fate Toward Osteoblast Differentiation Through alpha 1 beta 1 Integrin Signaling Pathway

Full text
Author(s):
Rosa, A. L. [1] ; Kato, R. B. [1] ; Castro Raucci, L. M. S. [1] ; Teixeira, L. N. [1] ; de Oliveira, F. S. [1] ; Bellesini, L. S. [1] ; de Oliveira, P. T. [1] ; Hassan, M. Q. [2] ; Beloti, M. M. [1]
Total Authors: 9
Affiliation:
[1] Univ Sao Paulo, Sch Dent Ribeirao Preto, Cell Culture Lab, BR-14040904 Ribeirao Preto, SP - Brazil
[2] Univ Alabama Birmingham, Sch Dent, Dept Oral & Maxillofacial Surg, Birmingham, AL 35294 - USA
Total Affiliations: 2
Document type: Journal article
Source: Journal of Cellular Biochemistry; v. 115, n. 3, p. 540-548, MAR 2014.
Web of Science Citations: 39
Abstract

The aim of our study was to investigate the osteoinductive potential of a titanium (Ti) surface with nanotopography, using mesenchymal stem cells (MSCs) and the mechanism involved in this phenomenon. Polished Ti discs were chemically treated with H2SO4/H2O2 to yield nanotopography and rat MSCs were cultured under osteogenic and non-osteogenic conditions on both nanotopography and untreated polished (control) Ti surfaces. The nanotopography increased cell proliferation and alkaline phosphatase (Alp) activity and upregulated the gene expression of key bone markers of cells grown under both osteogenic and non-osteogenic conditions. Additionally, the gene expression of 1 and 1 integrins was higher in cells grown on Ti with nanotopography under non-osteogeneic condition compared with control Ti surface. The higher gene expression of bone markers and Alp activity induced by Ti with nanotopography was reduced by obtustatin, an 11 integrin inhibitor. These results indicate that 11 integrin signaling pathway determines the osteoinductive effect of nanotopography on MSCs. This finding highlights a novel mechanism involved in nanosurface-mediated MSCs fate and may contribute to the development of new surface modifications aiming to accelerate and/or enhance the process of osseointegration. J. Cell. Biochem. 115: 540-548, 2014. (c) 2013 Wiley Periodicals, Inc. (AU)

FAPESP's process: 10/18395-3 - Large scale analisys of microRNAs in osteoblastic cells grown on titanium with nanotopography
Grantee:Márcio Mateus Beloti
Support Opportunities: Regular Research Grants
FAPESP's process: 10/19280-5 - Large Scale Analisys of MicroRNAs in Osteoblastic Cells Grown on Titanium with Nanotopography
Grantee:Rogério Bentes Kato
Support Opportunities: Scholarships in Brazil - Doctorate