PRODH Polymorphisms, Cortical Volumes and Thickness in Schizophrenia

Author(s):Show less -

Ota, Vanessa K. ^{[1, 2]} ; Bellucco, Fernanda T. ^[1] ; Gadelha, Ary ^{[3, 2]} ; Santoro, Marcos L. ^{[1, 2]} ; Noto, Cristiano ^{[3, 2]} ; Christofolini, Denise M. ^[4] ; Assuncao, Idaiane B. ^[3] ; Yamada, Karen M. ^[1] ; Ribeiro-dos-Santos, Andrea K. ^[5] ; Santos, Sidney ^[5] ; Mari, Jair J. ^[3] ; Smith, Marilia A. C. ^[1] ; Melaragno, Maria I. ^[1] ; Bressan, Rodrigo A. ^{[3, 2]} ; Sato, Joao R. ^{[6, 2]} ; Jackowski, Andrea P. ^[2] ; Belangero, Sintia I. ^{[1, 3, 2]}

Affiliation

^[1] Univ Fed Sao Paulo UNIFESP, Disciplina Genet, Dept Morfol & Genet, Sao Paulo - Brazil

^[2] Univ Fed Sao Paulo UNIFESP, LiNC, Sao Paulo - Brazil

^[3] Univ Fed Sao Paulo UNIFESP, Dept Psiquiatria, Sao Paulo - Brazil

^[4] Fac Med ABC FMABC, Dept Ginecol & Obstet, Disciplina Genet & Reprod Humana, Sao Paulo - Brazil

^[5] Fed Univ Para, Lab Genet Humana & Med, BR-66059 Belem, Para - Brazil

^[6] Univ Fed ABC, Ctr Math Computat & Cognit, Santo Andre - Brazil

• Document type: Journal article
• Source: PLoS One; v. 9, n. 2 FEB 3 2014.
• Web of Science Citations: 5

Abstract

Schizophrenia is a neurodevelopmental disorder with high heritability. Several lines of evidence indicate that the PRODH gene may be related to the disorder. Therefore, our study investigates the effects of 12 polymorphisms of PRODH on schizophrenia and its phenotypes. To further evaluate the roles of the associated variants in the disorder, we have conducted magnetic resonance imaging (MRI) scans to assess cortical volumes and thicknesses. A total of 192 patients were evaluated using the Structured Clinical Interview for DSM-IV (SCID), Positive and Negative Syndrome Scale (PANSS), Calgary Depression Scale, Global Assessment of Functioning (GAF) and Clinical Global Impression (CGI) instruments. The study included 179 controls paired by age and gender. The samples were genotyped using the real-time polymerase chain reaction (PCR), restriction fragment length polymorphism (RFLP)-PCR and Sanger sequencing methods. A sample of 138 patients and 34 healthy controls underwent MRI scans. One polymorphism was associated with schizophrenia (rs2904552), with the G-allele more frequent in patients than in controls. This polymorphism is likely functional, as predicted by PolyPhen and SIFT, but it was not associated with brain morphology in our study. In summary, we report a functional PRODH variant associated with schizophrenia that may have a neurochemical impact, altering brain function, but is not responsible for the cortical reductions found in the disorder. (AU)

• FAPESP's process: 11/50740-5 - Prevention in schizophrenia and bipolar disorder from neuroscience to the community: a multiphase, multimodal and translational platform for research and intervention
• Grantee: Rodrigo Affonseca Bressan
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 07/58736-1 - Investigation of 22q11.2 chromosomal region in the pathogenesis of cardiac alterations and schizophrenia
• Grantee: Marilia de Arruda Cardoso Smith
• Support Opportunities: Regular Research Grants