| Full text | |
| Author(s): |
De Morais Nunes, Edsel Renata
[1]
;
Zukurov, Jean Paulo
[1]
;
Maricato, Juliana Terzi
[2]
;
Araripe Sucupira, Maria Cecilia
[1]
;
Diaz, Ricardo Sobhie
[1]
;
Ramos Janini, Luiz Mario
[1, 2]
Total Authors: 6
|
| Affiliation: | [1] Univ Fed Sao Paulo, Dept Med, Sao Paulo - Brazil
[2] Univ Fed Sao Paulo, Dept Microbiol Immunol & Parasitol, Sao Paulo - Brazil
Total Affiliations: 2
|
| Document type: | Journal article |
| Source: | PLoS One; v. 9, n. 1 JAN 3 2014. |
| Web of Science Citations: | 1 |
| Abstract | |
The present study investigated the prevalence of HIV-1 multiple infections in a population composed by 47 patients under HAART failure and enrolled at the National DST/AIDS, Program, Ministry of Health, Brazil. Detection of multiple infections was done using a previously published RFLP assay for the HIV-1 protease gene, which is able of distinguishing between infections caused by a single or multiple HIV-1 subtypes. Samples with multiple infections were cloned, and sequence data submitted to phylogenetic analysis. We were able to identify 17 HIV-1 multiple infections out of 47 samples. Multiple infections were mostly composed by a mixture of recombinant viruses (94%), with only one case in which protease gene pure subtypes B and F were recovered. This is the first study that reports the prevalence of multiple infections and intersubtype recombinants in a population undergoing HAART in Brazil. Based on the data there was a steep increase of multiple infections after the introduction of the combined antiretroviral therapy in Brazil. Cases of multiple infections may be associated with HIV-1 genetic diversity through recombination allowing for the generation of viruses showing a combination of resistance mutations. (AU) | |
| FAPESP's process: | 09/11216-9 - Prevalence of Dual HIV-1 infections in virological failure individuals under HAART |
| Grantee: | Edsel Renata de Morais Nunes |
| Support Opportunities: | Scholarships in Brazil - Master |
| FAPESP's process: | 09/10915-0 - Prevalence of dual HIV-1 infections in virological failure individuals under HAART |
| Grantee: | Luiz Mário Ramos Janini |
| Support Opportunities: | Regular Research Grants |