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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Analysis of HIV-1 Protease Gene Reveals Frequent Multiple Infections Followed by Recombination among Drug Treated Individuals Living in Sao Paulo and Santos, Brazil

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Author(s):
De Morais Nunes, Edsel Renata [1] ; Zukurov, Jean Paulo [1] ; Maricato, Juliana Terzi [2] ; Araripe Sucupira, Maria Cecilia [1] ; Diaz, Ricardo Sobhie [1] ; Ramos Janini, Luiz Mario [1, 2]
Total Authors: 6
Affiliation:
[1] Univ Fed Sao Paulo, Dept Med, Sao Paulo - Brazil
[2] Univ Fed Sao Paulo, Dept Microbiol Immunol & Parasitol, Sao Paulo - Brazil
Total Affiliations: 2
Document type: Journal article
Source: PLoS One; v. 9, n. 1 JAN 3 2014.
Web of Science Citations: 1
Abstract

The present study investigated the prevalence of HIV-1 multiple infections in a population composed by 47 patients under HAART failure and enrolled at the National DST/AIDS, Program, Ministry of Health, Brazil. Detection of multiple infections was done using a previously published RFLP assay for the HIV-1 protease gene, which is able of distinguishing between infections caused by a single or multiple HIV-1 subtypes. Samples with multiple infections were cloned, and sequence data submitted to phylogenetic analysis. We were able to identify 17 HIV-1 multiple infections out of 47 samples. Multiple infections were mostly composed by a mixture of recombinant viruses (94%), with only one case in which protease gene pure subtypes B and F were recovered. This is the first study that reports the prevalence of multiple infections and intersubtype recombinants in a population undergoing HAART in Brazil. Based on the data there was a steep increase of multiple infections after the introduction of the combined antiretroviral therapy in Brazil. Cases of multiple infections may be associated with HIV-1 genetic diversity through recombination allowing for the generation of viruses showing a combination of resistance mutations. (AU)

FAPESP's process: 09/11216-9 - Prevalence of Dual HIV-1 infections in virological failure individuals under HAART
Grantee:Edsel Renata de Morais Nunes
Support Opportunities: Scholarships in Brazil - Master
FAPESP's process: 09/10915-0 - Prevalence of dual HIV-1 infections in virological failure individuals under HAART
Grantee:Luiz Mário Ramos Janini
Support Opportunities: Regular Research Grants