Green chromatographic fingerprinting: An environmentally friendly approach for the development of separation methods for fingerprinting complex matrices

Author(s)

Funari, Cristiano Soleo ^{[1, 2]} ; Carneiro, Renato Lajarim ^[3] ; Andrade, Andre Marques ^[1] ; Hilder, Emily Frances ^[2] ; Cavalheiro, Alberto Jose ^[1]

Affiliation

^[1] Sao Paulo State Univ, Inst Chem, Sao Paulo - Brazil

^[2] Univ Tasmania, Australian Ctr Res Separat Sci ACROSS, Sch Chem, Hobart, Tas 7001 - Australia

^[3] Univ Fed Sao Carlos, Dept Chem, BR-13560 Sao Carlos, SP - Brazil

• Document type: Journal article
• Source: JOURNAL OF SEPARATION SCIENCE; v. 37, n. 1-2, p. 37-44, JAN 2014.
• Web of Science Citations: 15

Abstract

A chromatographic fingerprint is a comprehensive method that reveals the distinctive pattern of peaks across the chromatogram for a given sample. It is considered an effective strategy to assess the identity and quality of herbal materials, as well as for the control of the quality of their derived products. HPLC is the most employed technique for these purposes and it is used routinely for quality control in industry. Hence, its impact on the environment should not be neglected. This work provides a rational and generic procedure to qualitatively fingerprint complex matrices. Resource- and time-saving experimental designs were selected; an alternative safer organic solvent was tested and a time-saving and innovative response entitled the green chromatographic fingerprinting response was developed and employed. This procedure was applied in the development of chromatographic fingerprints for extracts of Bauhinia forficata and Casearia sylvestris. Moreover, the response proposed here can be combined with a complementary metric available in the literature to compare methods using different solvents. According to this, the chromatographic fingerprints developed here using ethanol as the organic solvent provided a performance better than that of reference methods in which more harmful acetonitrile or methanol were employed. (AU)

• FAPESP's process: 12/15844-7 - A greener approach to the study of the green: sustainable liquid chromatography applied to the metabolite profile investigation of Brazilian medicinal plants
• Grantee: Cristiano Soleo de Funari
• Support Opportunities: Scholarships abroad - Research Internship - Post-doctor
• FAPESP's process: 10/16520-5 - Application of calibration chemometrics methods and multivariate curve resolution in Raman spectroscopy for qualitative, quantitative and polymorphism analyses in medicines
• Grantee: Renato Lajarim Carneiro
• Support Opportunities: Regular Research Grants