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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Characterization of Flavonoids and Phenolic Acids in Myrcia bella Cambess. Using FIA-ESI-IT-MSn and HPLC-PAD-ESI-IT-MS Combined with NMR

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Author(s):
Saldanha, Luiz L. [1] ; Vilegas, Wagner [2] ; Dokkedal, Anne L. [3]
Total Authors: 3
Affiliation:
[1] Univ Estadual Paulista UNESP, Inst Biosci, Dept Bot, BR-18618970 Sao Paulo - Brazil
[2] Univ Estadual Paulista UNESP, BR-11330900 Sao Paulo - Brazil
[3] Univ Estadual Paulista UNESP, Fac Sci, Dept Biol Sci, BR-17033360 Sao Paulo - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Molecules; v. 18, n. 7, p. 8402-8416, JUL 2013.
Web of Science Citations: 61
Abstract

The leaves of Myrcia DC. ex Guill species are used in traditional medicine and are also exploited commercially as herbal drugs for the treatment of diabetes mellitus. The present work aimed to assess the qualitative and quantitative profiles of M. bella hydroalcoholic extract, due to these uses, since the existing legislation in Brazil determines that a standard method must be developed in order to be used for quality control of raw plant materials. The current study identified eleven known flavonoid-O-glycosides and six acylated flavonoid derivatives of myricetin and quercetin, together with two kaempferol glycosides and phenolic acids such as caffeic acid, ethil galate, gallic acid and quinic acid. In total, 24 constituents were characterized, by means of extensive preparative chromatographic analyses, along with MS and NMR techniques. An HPLC-PAD-ESI-IT-MS and FIA-ESI-IT-MSn method were developed for rapid identification of acylated flavonoids, flavonoid-O-glycosides derivatives of myricetin and quercetin and phenolic acids in the hydroalcoholic M. bella leaves extract. The FIA-ESI-IT-MS techinique is a powerful tool for direct and rapid identification of the constituents after isolation and NMR characterization. Thus, it could be used as an initial method for identification of authentic samples concerning quality control of Myrcia spp extracts. (AU)

FAPESP's process: 09/52237-9 - Standardized herbal medicines for the treatment of chronic diseases
Grantee:Wagner Vilegas
Support Opportunities: BIOTA-FAPESP Program - Thematic Grants