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(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

In silico phylogenetic and virulence gene profile analyses of avian pathogenic Escherichia coli genome sequences

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Author(s):
Thaís C.G. Rojas [1] ; Renato P. Maluta [2] ; Luciano V. Koenigkan [3] ; Wanderley Dias da Silveira [2]
Total Authors: 4
Affiliation:
[1] Universidade de Campinas. Instituto de Biologia. Departamento de Genética, Evolução e Bioagentes - Brasil
[2] Univ Campinas Unicamp, Inst Biol, Dept Genet Evolucao & Bioagentes, BR-13083970 Campinas, SP - Brazil
[3] Embrapa Informat Agr, BR-13083886 Campinas, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Pesquisa Veterinária Brasileira; v. 34, n. 2, p. 129-133, 2014-02-00.
Abstract

Avian pathogenic Escherichia coli (APEC) infections are responsible for significant losses in the poultry industry worldwide. A zoonotic risk has been attributed to APEC strains because they present similarities to extraintestinal pathogenic E. coli (ExPEC) associated with illness in humans, mainly urinary tract infections and neonatal meningitis. Here, we present in silico analyses with pathogenic E. coli genome sequences, including recently available APEC genomes. The phylogenetic tree, based on multi-locus sequence typing (MLST) of seven housekeeping genes, revealed high diversity in the allelic composition. Nevertheless, despite this diversity, the phylogenetic tree was able to cluster the different pathotypes together. An in silico virulence gene profile was also determined for each of these strains, through the presence or absence of 83 well-known virulence genes/traits described in pathogenic E. coli strains. The MLST phylogeny and the virulence gene profiles demonstrated a certain genetic similarity between Brazilian APEC strains, APEC isolated in the United States, UPEC (uropathogenic E. coli) and diarrheagenic strains isolated from humans. This correlation corroborates and reinforces the zoonotic potential hypothesis proposed to APEC. (AU)

FAPESP's process: 12/04931-6 - Molecular characterization of avian pathogenic (APEC) and human uropathogenic (UPEC) Escherichia coli strains
Grantee:Wanderley Dias da Silveira
Support Opportunities: Regular Research Grants