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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Signaling Pathways Activation by Primary Endodontic Infectious Contents and Production of Inflammatory Mediators

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Author(s):
Martinho, Frederic C. [1, 2] ; Leite, Fabio R. M. [3] ; Chiesa, Wanderson M. M. [4] ; Nascimento, Gustavo G. [3] ; Feres, Magda [5] ; Gomes, Brenda P. F. A. [2]
Total Authors: 6
Affiliation:
[1] UNESP UNIV Estadual Paulista, Sao Jose dos Campos Dent Sch, Endodont Div, Dept Restorat Dent, Sao Jose Dos Campos, SP - Brazil
[2] Univ Estadual Campinas, Endodont Div, Dept Restorat Dent, UNICAMP, Piracicaba, SP - Brazil
[3] Univ Fed Pelotas, Sch Dent, UFPel, Dept Semiol & Clin, Periodont Div, Pelotas, RS - Brazil
[4] Amazonas State Univ, Endodont Div, Dept Restorat Dent, Manaus, Amazonas - Brazil
[5] Univ Fed Pelotas, Sch Dent, Dept Restorat Dent, Postgrad Div, UFPel, Pelotas, RS - Brazil
Total Affiliations: 5
Document type: Journal article
Source: JOURNAL OF ENDODONTICS; v. 40, n. 4, p. 484-489, APR 2014.
Web of Science Citations: 10
Abstract

Introduction: This study investigated the bacterial community involved in primary endodontic diseases, evaluated its ability to activate the macrophage Toll-like receptor 4 receptor through p38 mitogen-activated protein kinase (MAPK) and nuclear factor kappa B (NF-B-K) signaling pathways, and determined the levels of endotoxins and interleukins (interleukin {[}IL]-6 and -10) produced by endodontic content-stimulated macrophages. Methods: Samples were taken from 21 root canals by using sterile/apyrogenic paper points. Raw 264.7 macrophages were stimulated with root canal contents. Checkerboard DNA-DNA hybridization was used for bacterial analysis and the limulus amebocyte lysate assay for endotoxin measurement; p38 MAPK and NF-KB activation was determined by Western blot analysis. IL-6 and IL-10 were measured using the enzyme-linked immunosorbent assay. Results: Bacteria and endotoxins were detected in 100% of the samples (21/21). The most frequently observed species were Parvimonas micra (16121, 76%), Fusobacterium nudeatum ssp. nucleatum (15121, 71%), and Porphyromonas endodontalis (14121, 66%). Correlations were found between endotoxins and IL-6 and IL-10 (P <.05); p38 phosphorylation had a peak at 60 minutes, and NF-KB was quickly activated after 10 minutes of stimulation. Conclusions: It was concluded that the complex bacterial community was shown to be a potent activator of TLR-4 determined by the p38 MAPK and NF-KB signaling pathways, culminating in a high antigenicity against macrophages through the levels of IL-6 and IL-10, all significantly affected by endotoxin levels. (AU)

FAPESP's process: 10/19136-1 - Microbiological and endotoxins analysis in primarily infected root canals with apical periodontitis and their antigenic potencial against macrophages to produce IL1-beta e TNF-alfa during endodontic treatment
Grantee:Brenda Paula Figueiredo de Almeida Gomes
Support Opportunities: Regular Research Grants
FAPESP's process: 13/02402-9 - Microbiological and LPS investigation and their antigenic activity in teeth with pulpal necrosis and irreversible pulpitis in pro-inflammatory cytokine production
Grantee:Ariane Cássia Salustiano Marinho
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 10/17877-4 - Microbiological analysis and quantification of endotoxins and proinflammatory cytokines from primarily infected root canals with apical periodontitis
Grantee:Brenda Paula Figueiredo de Almeida Gomes
Support Opportunities: Regular Research Grants