| Full text | |
| Author(s): |
Elaine Monteiro Cardoso-Lopes
[1]
;
Daisy Maria Bentes de Paula
[2]
;
Fausto Erritto Barbo
[3]
;
Amanda de Souza
[4]
;
Cecília Terumi Teradaira Blatt
[5]
;
Luce Maria Brandão Torres
[6]
;
Maria Cláudia Marx Young
[7]
Total Authors: 7
|
| Affiliation: | [1] Instituto de Botânica. Seção de Fisiologia e Bioquímica de Plantas - Brasil
[2] Instituto de Botânica. Seção de Fisiologia e Bioquímica de Plantas - Brasil
[3] Instituto de Botânica. Seção de Fisiologia e Bioquímica de Plantas - Brasil
[4] Universidade de São Paulo. Instituto de Química. Laboratório de Química de Produtos Naturais - Brasil
[5] Instituto de Botânica. Seção de Fisiologia e Bioquímica de Plantas - Brasil
[6] Instituto de Botânica. Seção de Fisiologia e Bioquímica de Plantas - Brasil
[7] Instituto de Botânica. Seção de Fisiologia e Bioquímica de Plantas - Brasil
Total Affiliations: 7
|
| Document type: | Journal article |
| Source: | Rev. bras. Bot.; v. 32, n. 4, p. 819-825, 2009-12-00. |
| Abstract | |
Pera glabrata (Schott) Baill. was selected for this study after showing a preliminary positive result in a screening of Atlantic Forest plant species in the search for acetylcholinesterase inhibitors and antifungal compounds. The bioassays were conducted with crude ethanol extract of the leaves using direct bioautography method for acetylcholinesterase and antifungal activities. This extract was partitioned with hexane, chloroform and ethyl acetate solvents. The active chloroform fraction was submitted to silica gel chromatography column affording 12 groups. Caffeine, an alkaloid, which showed detection limits of 0.1 and 1.0 µg for anticholinesterasic and antifungal activities, respectively, was isolated from group nine. After microplate analyses, only groups four, nine, 10, 11 and 12 showed acetylcholinesterase inhibitory activity of 40% or higher. The group 12 was purified by preparative layer chromatography affording four sub-fractions. Two sub-fractions from this group were analyzed by gas chromatography-mass spectrometry and gas chromatography-flame ionization detector. The first sub-fraction showed anticholinesterasic activity and contained two major compounds: 9-hydroxy-4-megastigmen-3-one (84%) and caffeine (6%). The second sub-fraction presented five major compounds identified as 9-hydroxy-4-megastigmen-3-one, isololiolide, (-) loliolide, palmitic acid and lupeol and did not show activity. (AU) | |
| FAPESP's process: | 03/02176-7 - Conservation and sustainable use of the diversity from Cerrado and Atlantic Forest: chemical diversity and prospecting for potential drugs - phase II |
| Grantee: | Vanderlan da Silva Bolzani |
| Support Opportunities: | BIOTA-FAPESP Program - Thematic Grants |