Transferability and characterization of microsatellite markers in two Neotropical Ficus species

Author(s)

Nazareno, Alison Goncalves ^[1] ; Santinelo Pereira, Rodrigo Augusto ^[2] ; Feres, Juliana Massimino ^[3] ; Mestriner, Moacyr Antonio ^[3] ; Alzate-Marin, Ana Lilia ^[3]

Affiliation

^[1] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Programa Posgrad Biol Comparada, BR-14049 Ribeirao Preto - Brazil

^[2] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Biol, BR-14049 Ribeirao Preto - Brazil

^[3] Univ Sao Paulo, Dept Genet, Fac Med, Lab Genet Vegetal, BR-14049900 Ribeirao Preto - Brazil

• Document type: Journal article
• Source: GENETICS AND MOLECULAR BIOLOGY; v. 32, n. 3, p. 568-571, 2009.
• Web of Science Citations: 15

Abstract

Microsatellite markers were transferred and characterized for two Neotropical fig tree species, Ficus citrifolia and Ficus eximia. Our study demonstrated that microsatellite markers developed from different subgenera of Ficus can be transferred to related species. In the present case, 12 of the 15 primer pairs tested (80%) were successfully transferred to both of the above species. Eleven loci were polymorphic when tested across 60 F. citrifolia and 60 F. eximia individuals. For F. citrifolia, there were 4 to 15 alleles per locus, whereas expected heterozygosities ranged from 0.31 to 0.91. In the case of F. eximia, this was 2 to 12 alleles per locus and expected heterozygosities from 0.42 to 0.87. (AU)

• FAPESP's process: 04/10299-4 - Effects of the forest fragmentation in the State of São Paulo and other regions of the South and Southeast of the country in the functioning of populations of fig trees and in the fig-wasp mutualism of figs
• Grantee: Rodrigo Augusto Santinelo Pereira
• Support Opportunities: BIOTA-FAPESP Program - Young Investigators Grants