ERIC-PCR GENOTYPIC CHARACTERIZATION OF HAEMOPHILUS PARASUIS ISOLATED FROM BRAZILIAN SWINE

Author(s)

Moreno, Luisa Zanolli ^[1] ; Castilla, Karina Salvagni ^[1] ; Sena de Gobbi, Debora Dirani ^[1] ; Coutinho, Tania Alen ^[1] ; Porfida Ferreira, Thais Sebastiana ^[1] ; Moreno, Andrea Micke ^[1]

Affiliation

^[1] Univ Sao Paulo, Fac Med Vet & Zootecnia, Lab Sanidade Suina & Virol, Sao Paulo - Brazil

• Document type: Journal article
• Source: Brazilian Journal of Microbiology; v. 42, n. 4, p. 1420-1426, OCT-DEC 2011.
• Web of Science Citations: 5

Abstract

Haemophilus parasuis infection, known as Glasser's disease, is characterized by fibrinous polyserositis, arthritis and meningitis in piglets. Although traditional diagnosis is based on herd history, clinical signs, bacterial isolation and serotyping, the molecular-based methods are alternatives for species-specific tests and epidemiologic study. The aim of this study was to characterize H. parasuis strains isolated from different states of Brazil by serotyping, PCR and ERIC-PCR. Serotyping revealed serovar 4 as the most prevalent (24 %), followed by serovars 14 (14 %), 5 (12 %), 13 (8 %) and 2 (2 %), whereas 40 % of the strains were considered as non-typeable. From 50 strains tested 43 (86%) were positive to Group 1 vtaA gene that have been related to virulent strains of H. parasuis. ERIC-PCR was able to type isolates tested among 23 different patterns, including non-typeable strains. ERIC-PCR patterns were very heterogeneous and presented high similarity between strains of the same animal or farm origin. The results indicated ERIC-PCR as a valuable tool for typing H. parasuis isolates collected in Brazil. (AU)

• FAPESP's process: 07/04893-9 - Characterization of Haemophilus parasuis samples through amplified fragment length polymorphism (AFLP)
• Grantee: Luisa Zanolli Moreno
• Support Opportunities: Scholarships in Brazil - Scientific Initiation